{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:58:05Z","timestamp":1790776685624,"version":"4.1.0"},"reference-count":62,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"Natural Science Foundation of Xinjiang Uygur Autonomous Region","award":["2025D01B97"],"award-info":[{"award-number":["2025D01B97"]}]},{"name":"Innovative Research Group Project of the National Natural Science Foundation of China","award":["32160002"],"award-info":[{"award-number":["32160002"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,17]]},"abstract":"<jats:p>\n                    A novel bacterial strain, designated JK66\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from the rhizosphere soil of diseased\n                    <jats:italic>Cistanche<\/jats:italic>\n                    plants collected in Turpan, Xinjiang, China. Strain JK66\n                    <jats:sup>T<\/jats:sup>\n                    grew at 20\u201342 \u00b0C (optimum, 37 \u00b0C), pH 5.0\u201310.0 (optimum, pH 7.0), and with 0\u20133.0% (w\/v) NaCl (optimum, 2.0% (w\/v)). The strain was selected for taxonomic characterization because of its antagonistic activity against\n                    <jats:italic>Fusarium acuminatum<\/jats:italic>\n                    and its potential to produce indole-3-acetic acid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JK66\n                    <jats:sup>T<\/jats:sup>\n                    belongs to the genus\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    , showing the highest sequence similarity to\n                    <jats:italic>Pseudomonas borbori<\/jats:italic>\n                    R-20821\n                    <jats:sup>T<\/jats:sup>\n                    (98.70%), followed by\n                    <jats:italic>Pseudomonas cuatrocienegasensis<\/jats:italic>\n                    1N\n                    <jats:sup>T<\/jats:sup>\n                    (98.46%),\n                    <jats:italic>Pseudomonas segetis<\/jats:italic>\n                    CIP108523\n                    <jats:sup>T<\/jats:sup>\n                    (98.35%),\n                    <jats:italic>Pseudomonas marincola<\/jats:italic>\n                    KMM3042\n                    <jats:sup>T<\/jats:sup>\n                    (98.32%) and\n                    <jats:italic>Pseudomonas xionganensis<\/jats:italic>\n                    R-22-3w-18\n                    <jats:sup>T<\/jats:sup>\n                    (98.15%). The DNA G+C content of strain JK66\n                    <jats:sup>T<\/jats:sup>\n                    was 61.63%. The average nucleotide identity and digital DNA\u2013DNA hybridization values between strain JK66\n                    <jats:sup>T<\/jats:sup>\n                    and closely related type strains were 76.82\u201382.06% and 21.10\u201325.30%, respectively, which are below the threshold values for species delineation. Chemotaxonomic analyses showed that the respiratory quinone was ubiquinone Q-9. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid, unidentified glycolipid, unidentified phosphoglycolipid and two unidentified lipids and the predominant fatty acids (&gt;5%) were C\n                    <jats:sub>12:0<\/jats:sub>\n                    , C\n                    <jats:sub>12:0<\/jats:sub>\n                    3-OH, summed feature 3, C\n                    <jats:sub>16:0<\/jats:sub>\n                    and summed feature 8. On the basis of phenotypic, physiological, biochemical, phylogenetic and genomic evidence, strain JK66\n                    <jats:sup>T<\/jats:sup>\n                    represents a novel species within the genus\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    , for which the name\n                    <jats:italic>Pseudomonas cistanchae<\/jats:italic>\n                    sp. nov. is proposed. The type strain is JK66\n                    <jats:sup>T<\/jats:sup>\n                    (=CCTCC AB 2025123\n                    <jats:sup>T<\/jats:sup>\n                    =LMG 34229\n                    <jats:sup>T<\/jats:sup>\n                    ). The GenBank accession numbers for the 16S rRNA gene sequence and the whole-genome sequence are PX795502 and JBTLWN000000000, respectively.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007284","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:16:59Z","timestamp":1790767019000},"source":"Crossref","is-referenced-by-count":0,"title":["Pseudomonas cistanchae sp. nov., a novel plant-associated bacterium isolated from diseased Cistanche soil in Xinjiang, China"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3885-7471","authenticated-orcid":false,"given":"Siya","family":"Wu","sequence":"first","affiliation":[{"name":"Xinjiang Industry Technical College, Urumqi 830021, PR China"},{"name":"College of Life Sciences, Xinjiang Normal University\/Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Urumqi 830054, PR 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