{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:07:11Z","timestamp":1789052831393,"version":"build-2803163510"},"reference-count":66,"publisher":"Microbiology Society","issue":"9","funder":[{"DOI":"10.13039\/100014717","name":"National Science Fund for Excellent Young Scholars","doi-asserted-by":"crossref","award":["42222703"],"award-info":[{"award-number":["42222703"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["42277257"],"award-info":[{"award-number":["42277257"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guidance Project of Fujian Provincial Department of Science and Technology","award":["2025N0023 and 2025N0075"],"award-info":[{"award-number":["2025N0023 and 2025N0075"]}]},{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"crossref","award":["GJYS202306"],"award-info":[{"award-number":["GJYS202306"]}],"id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,10]]},"abstract":"<jats:p>\n                    Two novel aerobic, rod-shaped, motile bacterial strains, designated as sgz302134\n                    <jats:sup>T<\/jats:sup>\n                    and sgz301742\n                    <jats:sup>T<\/jats:sup>\n                    , were isolated from paddy soil in Fujian Province. Strains sgz302134\n                    <jats:sup>T<\/jats:sup>\n                    and sgz301742\n                    <jats:sup>T<\/jats:sup>\n                    shared the highest 16S rRNA gene sequence similarities with the type strains\n                    <jats:italic>Azospirillum isscasi<\/jats:italic>\n                    C340-1\n                    <jats:sup>T<\/jats:sup>\n                    (98.2%) and\n                    <jats:italic>Azospirillum thiophilum<\/jats:italic>\n                    DSM 21654\n                    <jats:sup>T<\/jats:sup>\n                    (97.4%), respectively. The phylogenetic tree based on 16S rRNA gene sequences showed that two strains clustered with members of the genus\n                    <jats:italic>Azospirillum<\/jats:italic>\n                    . Growth of strains sgz302134\n                    <jats:sup>T<\/jats:sup>\n                    and sgz301742\n                    <jats:sup>T<\/jats:sup>\n                    was observed at 10\u201345 \u00b0C, pH 5.0\u20139.5 and 0\u20130.5% (w\/v) NaCl and 15\u201337 \u00b0C, pH 6.0\u20139.0 and 0\u20131.0% (w\/v) NaCl, respectively. Strains sgz302134\n                    <jats:sup>T<\/jats:sup>\n                    and sgz301742\n                    <jats:sup>T<\/jats:sup>\n                    contained Q-10 as the main quinone. The main fatty acids (&gt;10%) of both strains were summed feature 2 (C\n                    <jats:sub>12 : 0<\/jats:sub>\n                    aldehyde), summed feature 3 (C\n                    <jats:sub>16 : 1<\/jats:sub>\n                    <jats:italic>\u03c9<\/jats:italic>\n                    7\n                    <jats:italic>c<\/jats:italic>\n                    and\/or C\n                    <jats:sub>16 : 1<\/jats:sub>\n                    <jats:italic>\u03c9<\/jats:italic>\n                    6\n                    <jats:italic>c<\/jats:italic>\n                    ), summed feature 8 (C\n                    <jats:sub>18 : 1<\/jats:sub>\n                    <jats:italic>\u03c9<\/jats:italic>\n                    7\n                    <jats:italic>c<\/jats:italic>\n                    and\/or C\n                    <jats:sub>18 : 1<\/jats:sub>\n                    <jats:italic>\u03c9<\/jats:italic>\n                    6\n                    <jats:italic>c<\/jats:italic>\n                    ) and C\n                    <jats:sub>16 : 0<\/jats:sub>\n                    . The genomic DNA G+C content of strains sgz302134\n                    <jats:sup>T<\/jats:sup>\n                    and sgz301742\n                    <jats:sup>T<\/jats:sup>\n                    was 68.4 and 68.3%, respectively. The digital DNA\u2013DNA hybridization and average nucleotide identity values between the two strains and their related reference strains were 27.8 and 87.4% and 22.0 and 84.3%, respectively. Both strains possessed\n                    <jats:italic>nif<\/jats:italic>\n                    genes\n                    <jats:italic>nifBDEHKN<\/jats:italic>\n                    . Based on the above results, these two strains represent two novel species of the genus\n                    <jats:italic>Azospirillum<\/jats:italic>\n                    , for which the names\n                    <jats:italic>Azospirillum mesophilum<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Azospirillum terrae<\/jats:italic>\n                    sp. nov. are proposed. The type strains are sgz302134\n                    <jats:sup>T<\/jats:sup>\n                    (=MCCC 1K09520\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 8840\n                    <jats:sup>T<\/jats:sup>\n                    ) and sgz301742\n                    <jats:sup>T<\/jats:sup>\n                    (=MCCC 1K09804\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 18149\n                    <jats:sup>T<\/jats:sup>\n                    ), respectively.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007289","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:18:56Z","timestamp":1789049936000},"source":"Crossref","is-referenced-by-count":0,"title":["Description of two nitrogen-fixing bacteria, Azospirillum mesophilum sp. nov. and Azospirillum terrae sp. nov., isolated from paddy soils"],"prefix":"10.1099","volume":"76","author":[{"given":"Xie-Feng","family":"Lu","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Gao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song-Zhuo","family":"Shi","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian 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