{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:42Z","timestamp":1792786182885,"version":"4.5.0"},"reference-count":59,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation M\u00e9diterran\u00e9e Infection"},{"name":"French National Research Agency","award":["ANR-10-IAHU-03"],"award-info":[{"award-number":["ANR-10-IAHU-03"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Regional Development Fund","award":["FEDER IHUPERF"],"award-info":[{"award-number":["FEDER IHUPERF"]}]},{"name":"Contrat Plan \u00c9tat-R\u00e9gion"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,8]]},"abstract":"<jats:p>\n                    Members of the genus\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    are widespread obligate intracellular bacteria with high medical and veterinary relevance. While\n                    <jats:italic>Rickettsia senegalensis<\/jats:italic>\n                    was previously isolated from cat fleas,\n                    <jats:italic>Ctenocephalides felis<\/jats:italic>\n                    , in Senegal, a comprehensive polyphasic characterization is required to validly publish its name. This study aims to provide the first detailed phenotypic, ultrastructural and genomic characterization of the type strain PU01-02\u1d40. Cultivation assays demonstrated that strain PU01-02\u1d40 grows aerobically in XTC-2, SF9 and LD652 cell lines at 28\u2009\u00b0C in a CO\n                    <jats:sub>2<\/jats:sub>\n                    -free atmosphere. Ultrastructural examination via scanning electron microscopy using a backscattered electron detector confirmed its free cytoplasmic localization and a typical rickettsial morphology characterized by rod-shaped cells ~1.5\u2009\u00b5m in length and 0.3\u2009\u00b5m in width. Whole-genome sequencing yielded a 1.62\u2009Mb genome with a G+C content of 33.2%, and three plasmids were detected. Genomic analyses confirmed its status as a distinct species, placing it within the transitional\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    group, specifically inside the\n                    <jats:italic>Rickettsia felis<\/jats:italic>\n                    cluster. A review of epidemiological data revealed that rickettsial genes identical to those of\n                    <jats:italic>R. senegalensis<\/jats:italic>\n                    had already been identified in several haematophagous arthropods, including fleas and ticks parasitizing various hosts such as cats, dogs, opossums and rodents worldwide. It has also been detected in cat tissues, suggesting a potential host-pathogen association.\n                  <\/jats:p>\n                  <jats:p>\n                    Based on these comprehensive characterization data, we formally propose\n                    <jats:italic>R. senegalensis<\/jats:italic>\n                    sp. nov. as a new species, with PU01-02\u1d40 (=CSUR R184\u1d40 =DSM 28250\u1d40) as the type strain.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007294","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:16:52Z","timestamp":1791476212000},"source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic, genomic and morphological characterization of Rickettsia senegalensis sp. nov., a novel Rickettsia species detected 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