{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:58:52Z","timestamp":1790769532146,"version":"4.1.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"Robert Koch Institut","award":["NRZ Borrelia"],"award-info":[{"award-number":["NRZ Borrelia"]}],"id":[{"id":"https:\/\/ror.org\/01k5qnb77","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Department of Agriculture, South Africa","award":["A149, A152"],"award-info":[{"award-number":["A149, A152"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,30]]},"abstract":"<jats:p>\n                    <jats:italic>Ornithodoros zumpti<\/jats:italic>\n                    is a soft tick species in eastern South Africa. In 1961, Zumpt and Organ described the transmission of a relapsing fever\n                    <jats:italic>Borrelia<\/jats:italic>\n                    species from\n                    <jats:italic>O. zumpti<\/jats:italic>\n                    to laboratory mice. The\n                    <jats:italic>Borrelia<\/jats:italic>\n                    species showed phenotypic differences to\n                    <jats:italic>Borrelia duttonii<\/jats:italic>\n                    and was designated\n                    <jats:italic>Borrelia tillae<\/jats:italic>\n                    Zumpt and Organ 1961 (Approved Lists 1980). At the German National Reference Center for\n                    <jats:italic>Borrelia<\/jats:italic>\n                    (NRZ), Oberschleissheim, a sample containing blood of an infected laboratory mouse labelled \u2018\n                    <jats:italic>Borrelia tillae<\/jats:italic>\n                    \u2019, was cultured. We sequenced PCR products of the 16S rRNA locus,\n                    <jats:italic>flaB<\/jats:italic>\n                    and MLST loci and generated whole-genome sequences using Pacific Biosciences technologies. The\n                    <jats:italic>Borrelia<\/jats:italic>\n                    16S rRNA amplification product from DNA of an\n                    <jats:italic>O. zumpti<\/jats:italic>\n                    colony was sequenced and compared to the 16S rRNA gene sequence from the culture at the NRZ. No identical matches to 16S rRNA gene,\n                    <jats:italic>flaB<\/jats:italic>\n                    or MLST sequences were found in BLASTn searches of the NCBI nucleotide database. In phylogenies of those sequences,\n                    <jats:italic>B. tillae<\/jats:italic>\n                    sequences clustered on their own branches. However, borrelial 16S rRNA gene sequences from an\n                    <jats:italic>O. zumpti<\/jats:italic>\n                    colony were identical to the sequence obtained from the cultured isolate. The genome of\n                    <jats:italic>B. tillae<\/jats:italic>\n                    consists of a linear chromosome, 5 linear and 11 circular plasmids (including the megaplasmid). Sequence comparison provides evidence that the recovered isolate (named \u2018Krampitz\u2019) differs from all\n                    <jats:italic>Borrelia<\/jats:italic>\n                    spp. for which sequence data exist. Identical 16S rRNA gene sequences from an\n                    <jats:italic>O. zumpti<\/jats:italic>\n                    colony to the cultured isolate strongly support the notion that we recovered\n                    <jats:italic>B. tillae<\/jats:italic>\n                    . Because there was no type strain available for\n                    <jats:italic>B. tillae<\/jats:italic>\n                    , we propose strain \u2018Krampitz\u2019 to be recognized as the type strain for the species according to rule 18f of the International Code of Nomenclature of Prokaryotes. The type strain, Krampitz\n                    <jats:sup>T<\/jats:sup>\n                    , has been deposited in two culture collections, DSMZ (=DSM 121361) and NCCB (=101126).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007295","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:16:52Z","timestamp":1790767012000},"source":"Crossref","is-referenced-by-count":0,"title":["Proposing a type strain for Borrelia tillae, a relapsing fever species from South Africa"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5304-9615","authenticated-orcid":false,"given":"Gabriele","family":"Margos","sequence":"first","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7768-4867","authenticated-orcid":false,"given":"Sabrina","family":"Hepner","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dunia","family":"Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Stockmeier","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Hizo-Teufel","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Hartberger","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5880-3709","authenticated-orcid":false,"given":"Frederic D. B.","family":"Schedel","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5238-7900","authenticated-orcid":false,"given":"Konstantin","family":"Kuleshov","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kraiczy","sequence":"additional","affiliation":[{"name":"Goethe University Frankfurt, University Hospital Frankfurt, Institute of Medical Microbiology and Infection Control, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Goethe University Frankfurt, University Hospital Frankfurt, Institute of Medical Microbiology and Infection Control, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3361-0780","authenticated-orcid":false,"given":"Tom G.","family":"Schwan","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben J.","family":"Mans","sequence":"additional","affiliation":[{"name":"Department of Life and Consumer Sciences, University of South Africa, Florida, South Africa"},{"name":"Department of Zoology and Entomology, University of the Free State, Bloemfontein, South Africa"},{"name":"Epidemiology, Parasites and Vectors, Agricultural Research Council \u2013 Onderstepoort Veterinary Research, Onderstepoort, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronel","family":"Pienaar","sequence":"additional","affiliation":[{"name":"Department of Zoology and Entomology, University of the Free State, Bloemfontein, South Africa"},{"name":"Epidemiology, Parasites and Vectors, Agricultural Research Council \u2013 Onderstepoort Veterinary Research, Onderstepoort, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1027-135X","authenticated-orcid":false,"given":"Dirk","family":"Metzler","sequence":"additional","affiliation":[{"name":"Division of Evolutionary Biology, Department of Biology, LMU Munich, Planegg-Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Sing","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[{"name":"German National Reference Center for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","first-page":"31","article-title":"Strains of spirochaetes isolated from Ornithodoros zumpti Heisch & Guggisberg, and from wild rats in the Cape Province. 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