{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:57:23Z","timestamp":1790611043639,"version":"4.1.0"},"reference-count":52,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","award":["R20F0003"],"award-info":[{"award-number":["R20F0003"]}],"id":[{"id":"https:\/\/ror.org\/05btm5233","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,25]]},"abstract":"<jats:p>\n                    A Gram-positive, non-spore-forming, rod-shaped actinobacterium, designated strain Le1\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from root nodules of\n                    <jats:italic>Lupinus luteus<\/jats:italic>\n                    cultivated in volcanic soils of southern Chile. Phylogenetic analysis based on the 16S rRNA gene placed strain Le1\u1d40 within the genus\n                    <jats:italic>Leifsonia<\/jats:italic>\n                    , showing the highest sequence similarity to\n                    <jats:italic>Leifsonia lichenia<\/jats:italic>\n                    and related species. However, genome-based analyses supported clear taxonomic separation. The draft genome of strain Le1\u1d40 is 4.03 Mb, with a DNA G+C content of 69.26 mol%. Average nucleotide identity and digital DNA\u2013DNA hybridization values between strain Le1\u1d40 and its closest relatives ranged from 88.5 to 80.8% and 32.8 to 21.3%, respectively, below the accepted species delineation thresholds. Phylogenomic reconstruction based on 92 conserved core genes confirmed that strain Le1\u1d40 forms a distinct and well-supported lineage within the genus. The predominant cellular fatty acids of strain Le1\u1d40 are anteiso-C15\u200a:\u200a0, anteiso-C17\u200a:\u200a0, iso-C16\u200a:\u200a0 and C16\u200a:\u200a0. Phenotypic characteristics differentiate strain Le1\u1d40 from its closest phylogenetic relatives. On the basis of polyphasic taxonomic evidence, strain Le1\u1d40 represents a novel species of the genus\n                    <jats:italic>Leifsonia<\/jats:italic>\n                    , for which the name\n                    <jats:italic>Leifsonia araucanica<\/jats:italic>\n                    sp. nov. is proposed. The type strain is Le1\u1d40 (=RGM 3741\u1d40=LMG 34089\u1d40). In addition, genome-based comparisons indicate that\n                    <jats:italic>Leifsonia naganoensis<\/jats:italic>\n                    Suzuki\n                    <jats:italic>et al<\/jats:italic>\n                    . 2000 should be regarded as a later heterotypic synonym of\n                    <jats:italic>Leifsonia aquatica<\/jats:italic>\n                    (ex Leifson 1962) Evtushenko\n                    <jats:italic>et al<\/jats:italic>\n                    . 2000.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007298","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:17:06Z","timestamp":1790608626000},"source":"Crossref","is-referenced-by-count":0,"title":["Leifsonia araucanica sp. nov., isolated from nodules of Lupinus luteus and reclassification of Leifsonia naganoensis as a later heterotypic synonym of Leifsonia aquatica"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9021-3213","authenticated-orcid":false,"given":"Grace","family":"Armijo-Godoy","sequence":"first","affiliation":[{"name":"CGNA (Agriaquaculture Nutritional Genomic Center), Las Heras 350, Temuco, 4781158, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Cottet","sequence":"additional","affiliation":[{"name":"Centro para la Resiliencia, Adaptaci\u00f3n y Mitigaci\u00f3n (CReAM), Universidad Mayor, Avenida Alemania 0281, Temuco, 4801043, Chile"},{"name":"Escuela de Agronom\u00eda, Facultad de Ciencias, Ingenier\u00eda y Tecnolog\u00eda, Universidad Mayor, Temuco 4801043, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annally","family":"Rupayan","sequence":"additional","affiliation":[{"name":"CGNA (Agriaquaculture Nutritional Genomic Center), Las Heras 350, Temuco, 4781158, 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