{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T10:43:14Z","timestamp":1792579394005,"version":"4.5.0"},"reference-count":57,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"National Natural Science Foundation of China","award":["32470135"],"award-info":[{"award-number":["32470135"]}]},{"name":"National Natural Science Foundation of China","award":["42177403"],"award-info":[{"award-number":["42177403"]}]},{"name":"National Natural Science Foundation of China","award":["32500105"],"award-info":[{"award-number":["32500105"]}]},{"name":"Natural Science Foundation of Shandong Province","award":["ZR2025MS450"],"award-info":[{"award-number":["ZR2025MS450"]}]},{"name":"Natural Science Foundation of Shandong Province","award":["ZR2025QC1451"],"award-info":[{"award-number":["ZR2025QC1451"]}]},{"name":"Open Foundation of Collaborative Innovation Center for Wetland Conservation and Green Development of Hebei Province","award":["2025hbxczx4-1"],"award-info":[{"award-number":["2025hbxczx4-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:p>\n                    Two actinobacterial strains with resistance to selenite and molybdate, designated as ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    , were isolated from the river sediment sample of Jinshui River in Xingtai City, Hebei Province, China. Based on 16S rRNA gene sequence analysis, strain ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    showed the highest sequence similarity to\n                    <jats:italic>Agromyces binzhouensis<\/jats:italic>\n                    CGMCC 4.7180\n                    <jats:sup>T<\/jats:sup>\n                    (98.5%), and strain ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    was most closely related to\n                    <jats:italic>Agromyces arachidis<\/jats:italic>\n                    JCM 19251\n                    <jats:sup>T<\/jats:sup>\n                    (99.1%),\n                    <jats:italic>Agromyces indicus<\/jats:italic>\n                    CCTCC AB 2011122\n                    <jats:sup>T<\/jats:sup>\n                    (98.8%),\n                    <jats:italic>Agromyces aurantiacus<\/jats:italic>\n                    DSM 14598\n                    <jats:sup>T<\/jats:sup>\n                    (98.6%),\n                    <jats:italic>Agromyces tropicus<\/jats:italic>\n                    JCM 15672\n                    <jats:sup>T<\/jats:sup>\n                    (98.4%),\n                    <jats:italic>Agromyces mediolanus<\/jats:italic>\n                    JCM 3346\n                    <jats:sup>T<\/jats:sup>\n                    (98.2%) and\n                    <jats:italic>Agromyces soli<\/jats:italic>\n                    JCM 16247\n                    <jats:sup>T<\/jats:sup>\n                    (98.0%). The 16S rRNA gene sequence similarity between strains ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    was 98.4%. Phylogenetic analysis based on 16S rRNA gene sequences and whole-genome sequences indicated that strains ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    belong to the genus\n                    <jats:italic>Agromyces<\/jats:italic>\n                    . The genome sizes of strains ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    were 3.9 and 4.0 Mb, with DNA G+C contents of 72 and 71.5 %, respectively. The average nucleotide identity and digital DNA\u2013DNA hybridization values with the closest relatives were below species delineation thresholds. The major fatty acids detected in strain ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    included anteiso-C\n                    <jats:sub>15 : 0<\/jats:sub>\n                    , iso-C\n                    <jats:sub>16 : 0<\/jats:sub>\n                    , anteiso-C\n                    <jats:sub>17 : 0<\/jats:sub>\n                    , C\n                    <jats:sub>16 : 0<\/jats:sub>\n                    and iso-C\n                    <jats:sub>15 : 0<\/jats:sub>\n                    . For strain ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    , the major cellular fatty acids were identified as anteiso-C\n                    <jats:sub>15 : 0<\/jats:sub>\n                    , anteiso-C\n                    <jats:sub>15 : 1<\/jats:sub>\n                    A, iso-C\n                    <jats:sub>16 : 0<\/jats:sub>\n                    and anteiso-C\n                    <jats:sub>17 : 0<\/jats:sub>\n                    . The major respiratory quinones of strains ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    were MK-11 and MK-12. The polar lipid profiles of both strains contained diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids. Both isolates could reduce Se(IV) to produce selenium-containing particles. Strain ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    was also capable of reducing Mo(VI) to molybdenum blue. According to the phenotypic, genotypic and phylogenetic data, strains ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    represent two novel species of the genus\n                    <jats:italic>Agromyces<\/jats:italic>\n                    , for which\n                    <jats:italic>Agromyces molybdatiresistens<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Agromyces selenitireducens<\/jats:italic>\n                    sp. nov. are proposed, respectively. The type strains are ZXT2-3\n                    <jats:sup>T<\/jats:sup>\n                    (=GDMCC 1.4963\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 37791\n                    <jats:sup>T<\/jats:sup>\n                    =MCCC 1K10748\n                    <jats:sup>T<\/jats:sup>\n                    ) and ZXT2-6\n                    <jats:sup>T<\/jats:sup>\n                    (=GDMCC 1.4965\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 37792\n                    <jats:sup>T<\/jats:sup>\n                    =MCCC 1K10749\n                    <jats:sup>T<\/jats:sup>\n                    ), respectively.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007320","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T10:16:33Z","timestamp":1792577793000},"source":"Crossref","is-referenced-by-count":0,"title":["Agromyces molybdatiresistens sp. nov. and Agromyces selenitireducens sp. nov., two 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