{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:25Z","timestamp":1787010565193,"version":"build-2736575974"},"reference-count":22,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:p>\n                    Human immunodeficiency virus type 1 (HIV-1) transmission often results from infection by a single transmitted\/founder (T\/F) virus. Here, we investigated the sensitivity of T\/F HIV-1 envelope glycoproteins (Envs) to microbicide candidate carbohydrate-binding agents (CBAs) griffithsin (GRFT), cyanovirin-N (CV-N) and\n                    <jats:italic>Galanthus nivalis<\/jats:italic>\n                    agglutinin (GNA), showing that T\/F Envs demonstrated different sensitivity to CBAs, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 0.006\u2009\u00b1\u20090.0003 to &gt;10\u200anM for GRFT, from 0.6\u2009\u00b1\u20090.2 to 28.9\u2009\u00b1\u20092.9\u200anM for CV-N and from 1.3\u2009\u00b1\u20090.2 to &gt;500\u200anM for GNA. We further revealed that deglycosylation at position 295 or 448 decreased the sensitivity of T\/F Env to GRFT, and at 339 to both CV-N and GNA. Mutation of all the three glcyans rendered a CBA-sensitive T\/F Env largely resistant to GRFT, indicating that the sensitivity of T\/F Env to GRFT is mainly determined by glycans at 295, 339 and 448. Our study identified specific T\/F Env residues associated with CBA sensitivity.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.000299","type":"journal-article","created":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T16:12:20Z","timestamp":1443197540000},"page":"3660-3666","source":"Crossref","is-referenced-by-count":11,"title":["Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin"],"prefix":"10.1099","volume":"96","author":[{"given":"Bodan","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukun","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR 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