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In this study, we have for the first time investigated the <jats:italic>in vivo<\/jats:italic> dissemination, safety and expression kinetics of rFPV post intranasal delivery using recombinant viruses expressing green fluorescent protein or mCherry. Both confocal microscopy of tissue sections using green fluorescent protein and <jats:italic>in vivo<\/jats:italic> Imaging System (IVIS) spectrum live animal and whole organ imaging studies using mCherry revealed that (i) the peak antigen expression occurs 12 to 24\u2009h post vaccination and no active viral gene expression is detected 96\u2009h post vaccination. (ii) The virus only infects the initial vaccination site (lung and nasal cavity) and does not disseminate to distal sites such as the spleen or gut. (iii) More importantly, rFPV does not cross the olfactory receptor neuron pathway. Collectively, our findings indicate that rFPV vector-based vaccines have all the hallmarks of a safe and effective mucosal delivery vector, suitable for clinical evaluation.<\/jats:p>","DOI":"10.1099\/jgv.0.000702","type":"journal-article","created":{"date-parts":[[2017,1,5]],"date-time":"2017-01-05T23:12:33Z","timestamp":1483657953000},"page":"496-505","source":"Crossref","is-referenced-by-count":11,"title":["Recombinant fowlpox virus vector-based vaccines: expression kinetics, dissemination and safety profile following intranasal delivery"],"prefix":"10.1099","volume":"98","author":[{"given":"David G","family":"Townsend","sequence":"first","affiliation":[{"name":"Molecular Mucosal Vaccine Immunology Group, Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra ACT 2601, 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