{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:59:51Z","timestamp":1770753591376,"version":"3.50.0"},"reference-count":34,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:p>The spike glycoprotein (S) of infectious bronchitis virus (IBV) comprises two subunits, S1 and S2. We have previously demonstrated that the S2 subunit of the avirulent Beau-R strain is responsible for its extended cellular tropism for Vero cells. Two recombinant infectious bronchitis viruses (rIBVs) have been generated; the immunogenic S1 subunit is derived from the IBV vaccine strain, H120, or the virulent field strain, QX, within the genetic background of Beau-R. The rIBVs BeauR-H120(S1) and BeauR-QX(S1) are capable of replicating in primary chicken kidney cell cultures and in Vero cells. These results demonstrate that rIBVs are able to express S1 subunits from genetically diverse strains of IBV, which will enable the rational design of a future generation of IBV vaccines that may be grown in Vero cells.<\/jats:p>","DOI":"10.1099\/jgv.0.001167","type":"journal-article","created":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T16:13:22Z","timestamp":1540397602000},"page":"1681-1685","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant infectious bronchitis viruses expressing heterologous S1 subunits: potential for a new generation of vaccines that replicate in Vero cells"],"prefix":"10.1099","volume":"99","author":[{"given":"Erica","family":"Bickerton","sequence":"first","affiliation":[{"name":"The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Dowgier","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Britton","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","first-page":"101","article-title":"Infectious bronchitis","volume-title":"Diseases of Poultry","author":"Cavanagh","year":"2003"},{"key":"R2","first-page":"161","article-title":"Avian coronavirus diseases and infectious bronchitis vaccine development","volume-title":"Coronaviruses: Molecular and Cellular Biology","author":"Britton","year":"2007"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.24.13804-13811.2004"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.2307\/1591367"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006055"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1080\/03079459308418945"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1637\/0005-2086(2002)046[0847:NIBIPC]2.0.CO;2"},{"key":"R8","doi-asserted-by":"crossref","first-page":"7607","DOI":"10.1128\/JVI.73.9.7607-7618.1999","article-title":"Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence","volume":"73","author":"S\u00e1nchez","year":"1999","journal-title":"J Virol"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01568-09"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-16-3-423"},{"key":"R11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/BF01348027","article-title":"Studies on avian infectious bronchitis virus (IBV). 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