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Screening of guano samples collected during the survey uncovered a bat coronavirus (<jats:italic>Betacoronavirus<\/jats:italic>) in<jats:italic>Cynopterus brachyotis<\/jats:italic>, commonly known as the lesser dog-faced fruit bat. Using a capture-enrichment sequencing platform, the full-length genome of the bat CoV was sequenced and found to be closely related to the bat coronavirus HKU9 species found in Leschenault\u2019s rousette discovered in the Guangdong and Yunnan provinces.<\/jats:p>","DOI":"10.1099\/jgv.0.001307","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T16:12:11Z","timestamp":1565971931000},"page":"1363-1374","source":"Crossref","is-referenced-by-count":35,"title":["Detection and characterization of a novel bat-borne coronavirus in Singapore using multiple molecular approaches"],"prefix":"10.1099","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3194-8799","authenticated-orcid":false,"given":"Xiao Fang","family":"Lim","sequence":"first","affiliation":[{"name":"Environmental Health Institute, National Environment Agency, Singapore"},{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2207-5615","authenticated-orcid":false,"given":"Chengfa Benjamin","family":"Lee","sequence":"additional","affiliation":[{"name":"Sungei Buloh Wetlands Reserve National Parks Board, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Marie","family":"Pascoe","sequence":"additional","affiliation":[{"name":"Sungei Buloh Wetlands Reserve National Parks Board, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choon Beng","family":"How","sequence":"additional","affiliation":[{"name":"Sungei Buloh Wetlands Reserve National Parks Board, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Chan","sequence":"additional","affiliation":[{"name":"Sungei Buloh Wetlands Reserve National Parks Board, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Hao","family":"Tan","sequence":"additional","affiliation":[{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5317-8983","authenticated-orcid":false,"given":"Xinglou","family":"Yang","sequence":"additional","affiliation":[{"name":"Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China"},{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8089-163X","authenticated-orcid":false,"given":"Zhengli","family":"Shi","sequence":"additional","affiliation":[{"name":"Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1370-2744","authenticated-orcid":false,"given":"October M.","family":"Sessions","sequence":"additional","affiliation":[{"name":"Saw Swee Hock School of Public Health, National University of Singapore, Singapore"},{"name":"Department of Pharmacy, National University of Singapore, Singapore"},{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-0535","authenticated-orcid":false,"given":"Lin-Fa","family":"Wang","sequence":"additional","affiliation":[{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2413-3863","authenticated-orcid":false,"given":"Lee Ching","family":"Ng","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4791-5024","authenticated-orcid":false,"given":"Danielle E.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Yap","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","first-page":"241","article-title":"Update: Outbreak of severe acute respiratory syndrome-worldwide, 2003. 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