{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:00:50Z","timestamp":1790704850227,"version":"4.1.0"},"reference-count":28,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SFB 1021"],"award-info":[{"award-number":["SFB 1021"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"crossref","award":["RAPID"],"award-info":[{"award-number":["RAPID"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"crossref","award":["Infect-ERA ESCential"],"award-info":[{"award-number":["Infect-ERA ESCential"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:p>The non-structural protein NSs is the main virulence factor of Rift Valley fever virus, a major zoonotic pathogen in Africa. NSs forms large aggregates in the nucleus and impairs induction of the antiviral type I IFN system by several mechanisms, including degradation of subunit p62 of the general RNA polymerase II transcription factor TFIIH. Here, we show that depletion of the nuclear pore protein Nup98 affects the nuclear import of NSs. Nonetheless, NSs was still able to degrade TFIIH-p62 under these conditions. Depletion of Nup98, however, had a negative effect on Rift Valley fever virus multiplication. Our data thus indicate that NSs utilizes Nup98 for import into the nucleus, but also plays a general role in the viral replication cycle.<\/jats:p>","DOI":"10.1099\/jgv.0.001347","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T16:12:34Z","timestamp":1572538354000},"page":"712-716","source":"Crossref","is-referenced-by-count":13,"title":["Nuclear pore protein Nup98 is involved in replication of Rift Valley fever virus and nuclear import of virulence factor NSs"],"prefix":"10.1099","volume":"101","author":[{"given":"Simone","family":"Lau","sequence":"first","affiliation":[{"name":"Institute for Virology, Philipps-University Marburg, D-35043 Marburg, Germany"},{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9737-337X","authenticated-orcid":false,"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Virology, Philipps-University Marburg, D-35043 Marburg, Germany"},{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, D-35392 Giessen, Germany"},{"name":"German Center for Infection Research (DZIF), partner sites Marburg and Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.05.009"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.01.011"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000765"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.9798-9806.2004"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045730"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3390\/v8060174"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02914-13"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02255-10"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00132-1"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0040013"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01549-12"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.09.003"},{"key":"R13","first-page":"193","article-title":"Nuclear localization signals (NLS)","volume":"3","author":"Boulikas","year":"1993","journal-title":"Crit Rev Eukaryot Gene Expr"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29236"},{"key":"R15","doi-asserted-by":"crossref","first-page":"5018","DOI":"10.1128\/JVI.73.6.5018-5025.1999","article-title":"The carboxy-terminal acidic domain of Rift Valley fever virus NSs protein is essential for the formation of filamentous structures but not for the nuclear localization of the protein","volume":"73","author":"Yadani","year":"1999","journal-title":"J Virol"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/nature11289"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2010.01415.x"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.12.054"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02509-14"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.6.11.1591"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0801"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.11.001"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90331-3"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067861"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00120-9"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.06.004"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610977104"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01670-07"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001347?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T03:22:30Z","timestamp":1702610550000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,1]]},"references-count":28,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,7,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jgv.0.001347","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,1]]}}}