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Pre-incubation of cells with <jats:italic>Maackia amurensis<\/jats:italic> lectin or <jats:italic>Sambucus nigra<\/jats:italic> agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both \u03b12,3-linked and \u03b12,6-linked sialic acids as attachment receptor. Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (<jats:sc>dl<\/jats:sc>-<jats:italic>threo<\/jats:italic>-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl <jats:italic>N<\/jats:italic>-acetyl-\u03b1-<jats:sc>d<\/jats:sc>-galactosaminide) indicated that <jats:italic>N<\/jats:italic>-linked, but not <jats:italic>O<\/jats:italic>-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid. Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment. We can conclude that terminal sialic acids attached to <jats:italic>N<\/jats:italic>-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.<\/jats:p>","DOI":"10.1099\/jgv.0.001429","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T21:14:08Z","timestamp":1590614048000},"page":"760-771","source":"Crossref","is-referenced-by-count":3,"title":["Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor"],"prefix":"10.1099","volume":"101","author":[{"given":"Hassan M.","family":"Mahsoub","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 205 Duck Pond Drive, Blacksburg, VA 24061-0442, USA"},{"name":"Poultry Production Department, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria 21545, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 205 Duck Pond Drive, Blacksburg, VA 24061-0442, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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