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Like severe acute respiratory syndrome coronavirus (SARS-CoV), which emerged in 2002 and caused a similar disease, SARS-CoV-2 is a betacoronavirus. Both viruses use human angiotensin-converting enzyme 2 (hACE2) as a receptor to enter cells. However, the SARS-CoV-2 spike (S) glycoprotein has a novel insertion that generates a putative furin cleavage signal and this has been postulated to expand the host range. Two low-passage (P) strains of SARS-CoV-2 (Wash1\u202f:\u202fP4 and Munich\u202f:\u202fP1) were cultured twice in Vero E6 cells and characterized virologically. Sanger and MinION sequencing demonstrated significant deletions in the furin cleavage signal of Wash1\u202f:\u202fP6 and minor variants in the Munich\u202f:\u202fP3 strain. Cleavage of the S glycoprotein in SARS-CoV-2-infected Vero E6 cell lysates was inefficient even when an intact furin cleavage signal was present. Indirect immunofluorescence demonstrated that the S glycoprotein reached the cell surface. Since the S protein is a major antigenic target for the development of neutralizing antibodies, we investigated the development of neutralizing antibody titres in serial serum samples obtained from COVID-19 human patients. These were comparable regardless of the presence of an intact or deleted furin cleavage signal. These studies illustrate the need to characterize virus stocks meticulously prior to performing either\n                    <jats:italic>in vitro<\/jats:italic>\n                    or\n                    <jats:italic>in vivo<\/jats:italic>\n                    pathogenesis studies.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.001481","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T12:14:58Z","timestamp":1598012098000},"page":"1156-1169","source":"Crossref","is-referenced-by-count":129,"title":["SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patients"],"prefix":"10.1099","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4506-7842","authenticated-orcid":false,"given":"William B.","family":"Klimstra","sequence":"first","affiliation":[{"name":"Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, 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