{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:46:46Z","timestamp":1792655206929,"version":"4.5.0"},"reference-count":30,"publisher":"Microbiology Society","issue":"3","funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People\u2032s Republic of China","doi-asserted-by":"crossref","award":["2018ZX10102001"],"award-info":[{"award-number":["2018ZX10102001"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"crossref","award":["2018ZX10305-410-003-002"],"award-info":[{"award-number":["2018ZX10305-410-003-002"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"<jats:p>Fibre is the viral protein that mediates the attachment and infection of adenovirus to the host cell. Fowl adenovirus 4 (FAdV-4) possesses two different fibre trimers on each penton capsomere, and roles of the separate fibres remain elusive. Here, we attempted to investigate the function of FAdV-4 fibres by using reverse genetics approaches. Adenoviral plasmids carrying fiber1 or fiber2 mutant genes were constructed and used to transfect chicken LMH cells. Fiber1-mutated recombinant virus could not be rescued. Such defective phenotype was complemented when a fiber1-bearing helper plasmid was included for co-transfection. The infection of fiber-intact FAdV-4 (FAdV4-GFP) to LMH cells could be blocked with purified fiber1 knob protein in a dose-dependent manner, while purifed fiber2 knob had no such function. On the contrary, fiber2-mutated FAdV-4, FAdV4XF2-GFP, was successfully rescued. The results of one-step growth curves showed that proliferative capacity of FAdV4XF2-GFP was 10 times lower than that of the control FAdV4-GFP. FAdV4XF2-GFP also caused fewer deaths of infected chicken embryos than FAdV4-GFP did, which resulted from poorer virus replication <jats:italic>in vivo<\/jats:italic>. These data illustrated that fiber1 mediated virus adsorption and was essential for FAdV-4, while fiber2 was dispensable although it significantly contributed to the virulence.<\/jats:p>","DOI":"10.1099\/jgv.0.001559","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T14:17:56Z","timestamp":1614176276000},"source":"Crossref","is-referenced-by-count":20,"title":["Fiber1, but not fiber2, is the essential fiber gene for fowl adenovirus 4 (FAdV-4)"],"prefix":"10.1099","volume":"102","author":[{"given":"Xiaohui","family":"Zou","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yejing","family":"Rong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo 315010, PR China"},{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Guo","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhe","family":"Hou","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingyu","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China"},{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Hung","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5767-401X","authenticated-orcid":false,"given":"Zhuozhuang","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, PR China"},{"name":"Chinese Center for Disease Control and Prevention-Wuhan Institute of Virology, Chinese Academy of Sciences Joint Research Center for Emerging Infectious Diseases and Biosafety, Wuhan 430071, PR China"},{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.3390\/v4050847"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-092818-015550"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-019-00645-2"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1009"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.05.020"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0504"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/BF01315225"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(71)90175-9"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-007-0194-9"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.11.003"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-6-1465"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2012.04.005"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.10.7909-7915.1998"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.05.011"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82845-0"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1969-7"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex087"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2016.115"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.3390\/v12030301"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.05.010"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1293-z"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.3791\/52065"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.12.056"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.030064-0"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.01.028"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.03.031"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.12.041"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2017.12.003"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2010.08.006"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0203-1"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001559?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T12:16:50Z","timestamp":1617193010000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001559"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,1]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jgv.0.001559","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,1]]}}}