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C exists as a homodimer that forms via hydrophobic interactions between the \u03b12 and \u03b14 helices of monomers. To identify C region(s) important for virus particle production, a complementation system was employed in which single-round infectious particles are generated by <jats:italic>trans<\/jats:italic>-encapsidation of a viral C-deleted genome by recombinant C expressed in mosquito cells. Mutants harbouring a complete \u03b13 deletion, or a dual Ile65-\/Trp69-to-Ala substitution in the \u03b13 helix, exhibited reduced production of infectious virus. Unexpectedly, higher proportions of oligomeric C were detected in cells expressing both mutated forms as compared with the wild-type counterpart, indicating that the \u03b13 helix, through its internal hydrophobic residues, may down-modulate oligomerization of C during particle formation. Compared with wild-type C, the double Ile65-\/Trp69 to Ala mutations appeared to hamper viral infectivity but not C and genomic RNA incorporation into the pseudo-infectious virus particles, suggesting that increased C oligomerization may impair DENV replication at the cell entry step.<\/jats:p>","DOI":"10.1099\/jgv.0.001635","type":"journal-article","created":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T17:59:39Z","timestamp":1629395979000},"source":"Crossref","is-referenced-by-count":1,"title":["Increased capsid oligomerization is deleterious to dengue virus particle production"],"prefix":"10.1099","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9338-774X","authenticated-orcid":false,"given":"Sutha","family":"Sangiambut","sequence":"first","affiliation":[{"name":"Medical Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency, Bangkok 12120, Thailand"},{"name":"Siriraj Center of 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