{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T22:10:16Z","timestamp":1784153416302,"version":"3.55.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"applied basic research project of sichuan province, china","award":["2020YJ0247"],"award-info":[{"award-number":["2020YJ0247"]}]},{"DOI":"10.13039\/501100001809","name":"the national natural science foundation of china","doi-asserted-by":"crossref","award":["31772766"],"award-info":[{"award-number":["31772766"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"fundamental research funds for the central universities of southwest minzu university","award":["2021NYYXS85"],"award-info":[{"award-number":["2021NYYXS85"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,15]]},"abstract":"<jats:p>Feline coronavirus (FCoV) is the causative agent of feline infectious peritonitis and diarrhoea in kittens worldwide. In this study, a total of 173 feline diarrhoeal faecal and ascetic samples were collected from 15 catteries and six veterinary hospitals in southwest China from 2017 to 2020. FCoV was detected in 80.35\u200a% (139\/173) of the samples using the RT-nPCR method; these included infections with 122 type I FCoV and 57 type II FCoV. Interestingly, 51\u2009cases had co-infection with types I and II, the first such report in mainland China. To further analyse the genetic diversity of FCoV, we amplified 23 full-length spike (S) genes, including 18 type I and five type II FCoV. The type I FCoV and type II FCoV strains shared 85.5\u201398.7% and 97.4\u201398.9% nucleotide (nt) sequence identities between one another, respectively. The N-terminal domain (NTD) of 23 FCoV strains showed a high degree of variation (73.6\u201380.3\u200a%). There was six type I FCoV strains with two amino acid insertions (159HL160) in the NTD. In addition, 18 strains of type I FCoV belonged to the Ie cluster, and five strains of type II FCoV were in the IIb cluster based on phylogenetic analysis. Notably, it was first time that two type I FCoV strains had recombination in the NTD, and the recombination regions was located 140\u2013857 nt of the S gene. This study constitutes a systematic investigation of the current infection status and molecular characteristics of FCoV in southwest China.<\/jats:p>","DOI":"10.1099\/jgv.0.001654","type":"journal-article","created":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T15:17:13Z","timestamp":1631719033000},"source":"Crossref","is-referenced-by-count":21,"title":["Prevalence and molecular characteristics of feline coronavirus in southwest China from 2017 to 2020"],"prefix":"10.1099","volume":"102","author":[{"given":"Qun","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Key laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu 610041, PR China"},{"name":"College of 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