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However, experimental studies of candidate antivirals may be complicated by varying amounts and different forms of the protein E in the virus samples. Viral particles with different conformations and arrangements of the protein E are produced during the replication cycle of flaviviruses, including mature, partially mature, and immature forms, as well as subviral particles lacking genomic RNA. The immature forms are known to be abundant in the viral population. We obtained immature virion preparations of TBEV, characterized them by RT<jats:italic>-q<\/jats:italic>PCR, and assessed<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>infectivity of the residual mature virions in the immature virus samples. Analysis of the \u03b2-OG pocket structure on the immature virions confirmed the possibility of binding of adamantylmethyl esters of 5-aminoisoxazole-3-carboxylic acid in the pocket. We demonstrated that the antiviral activity of these compounds in plaque reduction assay is significantly reduced in the presence of immature TBEV particles.<\/jats:p>","DOI":"10.1099\/jgv.0.001658","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T18:17:23Z","timestamp":1632248243000},"source":"Crossref","is-referenced-by-count":5,"title":["Effect of immature tick-borne encephalitis virus particles on antiviral activity of 5-aminoisoxazole-3-carboxylic acid adamantylmethyl esters"],"prefix":"10.1099","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5668-3603","authenticated-orcid":false,"given":"Ksenia K.","family":"Tuchynskaya","sequence":"first","affiliation":[{"name":"FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), Moscow 108819, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3457-8367","authenticated-orcid":false,"given":"Anastasiia 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