{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:06:10Z","timestamp":1770977170898,"version":"3.50.1"},"reference-count":29,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,18]]},"abstract":"<jats:p>The polymerase acidic (PA) I38T substitution is a dominant marker of resistance to baloxavir. We evaluated the impact of I38T on the fitness of a contemporary influenza A(H3N2) virus. Influenza A\/Switzerland\/9715293\/2013 (H3N2) wild-type (WT) virus and its I38T mutant were rescued by reverse genetics. Replication kinetics were compared using ST6GalI-MDCK and A549 cells and infectivity\/contact transmissibility were evaluated in guinea pigs. Nasal wash (NW) viral titres were determined by TCID50 ml<jats:sup>\u22121<\/jats:sup> in ST6GalI-MDCK cells. Competition experiments were performed and the evolution of viral population was assessed by droplet digital RT-PCR. I38T did not alter <jats:italic>in vitro<\/jats:italic> replication. I38T induced comparable titres vs the WT in guinea pigs NWs and the two viruses transmitted equally by direct contact. However, a 50\u200a%:50\u200a% mixture inoculum evolved to mean WT\/I38T ratios of 71\u200a%:29\u200a% and 66.4\u200a%:33.6\u200a% on days 4 and 6 p.i., respectively. Contemporary influenza A(H3N2)-I38T PA variants may conserve a significant level of viral fitness.<\/jats:p>","DOI":"10.1099\/jgv.0.001659","type":"journal-article","created":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T21:42:07Z","timestamp":1634593327000},"source":"Crossref","is-referenced-by-count":6,"title":["Replication and transmission of an influenza A(H3N2) virus harboring the polymerase acidic I38T substitution, in guinea pigs."],"prefix":"10.1099","volume":"102","author":[{"given":"Zeineb","family":"Mhamdi","sequence":"first","affiliation":[{"name":"Research Center in Infectious Diseases of the \u201cCHU de Qu\u00e9bec\u201d and Laval University, Qu\u00e9bec City, Qu\u00e9bec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2697-9731","authenticated-orcid":false,"given":"Julie","family":"Carbonneau","sequence":"additional","affiliation":[{"name":"Research Center in Infectious Diseases of the \u201cCHU de 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