{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T04:46:49Z","timestamp":1772513209819,"version":"3.50.1"},"reference-count":83,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["DP190102048"],"award-info":[{"award-number":["DP190102048"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,21]]},"abstract":"<jats:p>Mosquito-borne flaviviruses are responsible for viral infections and represent a considerable public health burden. <jats:italic>Aedes aegypti<\/jats:italic> is the principal vector of dengue virus (DENV), therefore understanding the intrinsic virus\u2013host interactions is vital, particularly in the presence of the endosymbiont <jats:italic>Wolbachia,<\/jats:italic> which blocks virus replication in mosquitoes. Here, we examined the transcriptional response of <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1005\" xlink:type=\"simple\">Wolbachia<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>-transinfected <jats:italic>Ae. aegypti<\/jats:italic> Aag2 cells to DENV infection. We identified differentially expressed immune genes that play a key role in the activation of anti-viral defence such as the Toll and immune deficiency pathways. Further, genes encoding cytosine and N<jats:sup>6<\/jats:sup>-adenosine methyltransferases and SUMOylation, involved in post-transcriptional modifications, an antioxidant enzyme, and heat-shock response were up-regulated at the early stages of DENV infection and are reported here for the first time. Additionally, several long non-coding RNAs were among the differentially regulated genes. Our results provide insight into <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1005\" xlink:type=\"simple\">Wolbachia<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>-transinfected <jats:italic>Ae. aegypti<\/jats:italic>\u2019s initial virus recognition and transcriptional response to DENV infection.<\/jats:p>","DOI":"10.1099\/jgv.0.001694","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T15:17:18Z","timestamp":1641827838000},"source":"Crossref","is-referenced-by-count":7,"title":["Transcriptional response of Wolbachia-transinfected Aedes aegypti mosquito cells to dengue virus at early stages of 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