{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:10:06Z","timestamp":1747181406183,"version":"3.40.5"},"reference-count":30,"publisher":"Microbiology Society","issue":"3","funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"crossref","award":["2021M691170"],"award-info":[{"award-number":["2021M691170"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China National Postdoctoral Program for Innovative Talents","award":["BX2021109"],"award-info":[{"award-number":["BX2021109"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31872451"],"award-info":[{"award-number":["31872451"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,2]]},"abstract":"<jats:p>Rabies, caused by rabies lyssavirus (RABV), is a fatal disease among humans and almost all warm-blooded animals. Our previous study showed that the long non-coding RNA (lncRNA) EZH2 degradation-associated lncRNA (EDAL) effectively inhibits RABV infection both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> by degrading EZH2 and promoting the transcription of an antiviral gene, <jats:italic>Pcp4l1<\/jats:italic>. Herein, we found that recombinant RABV expressing EDAL (rRABV-EDAL) restricts RABV replication in primary granule neurons but not in primary cortical neurons or astrocytes. Further study revealed that EDAL induced EZH2 protein degradation and thereby decreased trimethylation of lysine 27 on the histone 3 (H3K27me3) level in granule neuron cells but not in cortical neurons or astrocytes. Furthermore, rRABV-EDAL infection induces more <jats:italic>Pcp4l1<\/jats:italic> mRNA transcription in granule neurons, while there are almost no obvious changes in cortical neurons or astrocytes. Consistently, compared with the parent virus RABV, reduced pathogenicity of rRABV-EDAL was observed in mice post-intranasal infection but not intramuscular infection. These results suggest that the lncRNA EDAL restricts RABV replication in a cell-specific and infection route-dependent manner.<\/jats:p>","DOI":"10.1099\/jgv.0.001725","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T15:16:26Z","timestamp":1646234186000},"source":"Crossref","is-referenced-by-count":1,"title":["lncRNA EDAL restricts rabies lyssavirus replication in a cell-specific and infection route-dependent manner"],"prefix":"10.1099","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2738-5804","authenticated-orcid":false,"given":"Baokun","family":"Sui","sequence":"first","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanjuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanchun","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6293-9329","authenticated-orcid":false,"given":"Zhen F.","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0569-8105","authenticated-orcid":false,"given":"Ling","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China"},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1511-3"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.12.009"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlaa054"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/s10072-020-04575-3"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1080\/13550280591002450"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.33588\/rn.6207.2016152"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.2174\/157016206777709500"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.supplement_4.s771"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-100114-055157"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)00141-6"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.03.014"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06862-2"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.2174\/0929866525666180809142326"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-11-0209"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706729105"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1101\/gr.217463.116"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-120213-092323"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2521"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1321"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03138"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-02150-9"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3108"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02050-15"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00109.2017"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-020-01074-6"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020412"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05075-11"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.18.11801-11812.2005"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.02011"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001016"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001725?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T15:16:30Z","timestamp":1646234190000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,2]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3,2]]}},"URL":"https:\/\/doi.org\/10.1099\/jgv.0.001725","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"type":"print","value":"0022-1317"},{"type":"electronic","value":"1465-2099"}],"subject":[],"published":{"date-parts":[[2022,3,2]]}}}