{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:10:32Z","timestamp":1787062232431,"version":"build-2736575974"},"reference-count":42,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T00:00:00Z","timestamp":1651190400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National SCience and Technology Major project of China","award":["2017ZX10302201001005"],"award-info":[{"award-number":["2017ZX10302201001005"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81873962"],"award-info":[{"award-number":["81873962"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81671998"],"award-info":[{"award-number":["81671998"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["91542207"],"award-info":[{"award-number":["91542207"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["91842309"],"award-info":[{"award-number":["91842309"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program","award":["2017BT01S131"],"award-info":[{"award-number":["2017BT01S131"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,29]]},"abstract":"<jats:p>\n                    The expression of various forms of hepatitis B virus (HBV) surface proteins regulates the release of mature virion, but whether they affect the release of other incomplete viral particles, such as naked capsid, is not clear. Here, by stable overexpression of large or middle\/small hepatitis B surface proteins (LHBs, M\/SHBs) in HepAD38 cells, we evaluated their effects on the release of complete and incomplete viral particles. Overproduction of LHBs inhibited the release of all surface proteins, which increased the ratio of naked capsids\/virions. This effect was accompanied by the elevated extracellular HBV RNA. On the other hand, overexpression of M\/SHBs greatly improved the secretion of enveloped viral and subviral particles.\n                    <jats:italic>In situ<\/jats:italic>\n                    visualization of viral DNA and LHBs revealed intracellular retention of mature virions when LHBs were overexpressed. These results indicate that the molecular decision on secretion of enveloped or unenveloped viral particles is modulated by the intracellular ratio of large, middle and small surface antigens. This mechanism may be relevant in the progression and resolution of HBV-induced chronic liver disease.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.001733","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T11:04:40Z","timestamp":1650366280000},"source":"Crossref","is-referenced-by-count":9,"title":["The role of hepatitis B virus surface proteins in regulating the maturation and secretion of complete and incomplete virions"],"prefix":"10.1099","volume":"103","author":[{"given":"Mingzhu","family":"Xu","sequence":"first","affiliation":[{"name":"Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijie","family":"Tang","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0268-4891","authenticated-orcid":false,"given":"Zhenghong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China"},{"name":"Centre for Research in Therapeutic Solutions, Faculty of Science and Technology, University of Canberra, ACT, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-017-2765-2"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.12.007"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00272-18"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002255"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.3390\/v9030056"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90364-8"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005123"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12398"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6700-1_15"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00798-18"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.05.029"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30325"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.02.007"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8278(91)90036-b"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01305-21"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.3.1059"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.60.3.880-887.1986"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.9.10.4459-4466.1989"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1126\/science.3787251"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90770-8"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.8.1715"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI83339"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2017.84"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.61.3.782-786.1987"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.10.7387-7392.1997"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02627-10"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27050"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00049"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00905-09"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-313811"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.12.003"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy119"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.08.039"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008945"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.07.019"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.02.008"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01363.x"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704000104"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02741-06"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.19.6909"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01557.x"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12398"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001733?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T16:28:55Z","timestamp":1702571335000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001733"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,29]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4,29]]}},"URL":"https:\/\/doi.org\/10.1099\/jgv.0.001733","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,29]]}}}