{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T07:44:51Z","timestamp":1781855091447,"version":"3.54.5"},"reference-count":47,"publisher":"Microbiology Society","issue":"5","funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"crossref","award":["NV19-05-00457"],"award-info":[{"award-number":["NV19-05-00457"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"crossref","award":["20-14325S"],"award-info":[{"award-number":["20-14325S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"crossref","award":["20-30500S"],"award-info":[{"award-number":["20-30500S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,4]]},"abstract":"<jats:p>Extensive axonal and neuronal loss is the main cause of severe manifestations and poor outcomes in tick-borne encephalitis (TBE). Phosphorylated neurofilament heavy subunit (pNF-H) is an essential component of axons, and its detection in cerebrospinal fluid (CSF) or serum can indicate the degree of neuroaxonal damage. We examined the use of pNF-H as a biomarker of neuroaxonal injury in TBE. In 89\u2009patients with acute TBE, we measured CSF levels of pNF-H and 3 other markers of brain injury (glial fibrillary acidic protein, S100B and ubiquitin C-terminal hydrolase L1) and compared the results to those for patients with meningitis of other aetiology and controls. Serum pNF-H levels were measured in 80\u2009patients and compared with findings for 90 healthy blood donors. TBE patients had significantly (<jats:italic>P<\/jats:italic>&lt;0.001) higher CSF pNF-H levels than controls as early as hospital admission. Serum pNF-H concentrations were significantly higher in samples from TBE patients collected at hospital discharge (<jats:italic>P<\/jats:italic>&lt;0.0001) than in controls. TBE patients with the highest peak values of serum pNF-H, exceeding 10\u200a000\u2009pg\u2009ml<jats:sup>\u22121<\/jats:sup>, had a very severe disease course, with coma or tetraplegia. Patients requiring intensive care had significantly higher serum pNF-H levels than other TBE patients (<jats:italic>P<\/jats:italic>&lt;0.01). Elevated serum pNF-H values were also observed in patients with incomplete recovery (<jats:italic>P<\/jats:italic>&lt;0.05). Peak serum pNF-H levels correlated positively with the duration of hospitalization (<jats:italic>P<\/jats:italic>=0.005). Measurement of pNF-H levels in TBE patients might be useful for assessing disease severity and determining prognosis.<\/jats:p>","DOI":"10.1099\/jgv.0.001743","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T15:16:16Z","timestamp":1651677376000},"source":"Crossref","is-referenced-by-count":5,"title":["Serum and cerebrospinal fluid phosphorylated neurofilament heavy subunit as a marker of neuroaxonal damage in tick-borne encephalitis"],"prefix":"10.1099","volume":"103","author":[{"given":"Andrea","family":"Fortova","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, CZ-62100 Brno, Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaclav","family":"H\u00f6nig","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, CZ-37005 Ceske Budejovice, Czechia"},{"name":"Department of 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