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Numerous studies have examined the antitumour effects of combinational use of an OAd and anticancer agents; however, few chemical compounds enhancing OAd infection have been reported. In this study, we screened a food and drug administration (FDA)-approved drug library containing 1134 small chemical compounds to identify chemical compounds that enhance OAd replication in human tumour cells. We found that domperidone, a dopamine D2 receptor antagonist, significantly enhanced the replication of an OAd in human tumour cells, including human pancreatic tumour cells, by two\u2013fivefold, resulting in improvement of OAd-mediated tumour cell killing activities. The E1A mRNA levels were significantly increased in domperidone-pre-treated cells following OAd infection, which contributed to the promotion of OAd replication. However, mRNA levels of the dopamine D2 receptor (DRD2), which is known to be a target molecule of domperidone, were undetectable in most of the tumour cells by real-time reverse transcription (RT)-PCR analysis, indicating that domperidone promoted OAd replication by acting on a molecule other than DRD2. This study provides important clues for the improvement of OAd-mediated cancer therapy.<\/jats:p>","DOI":"10.1099\/jgv.0.001752","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T16:17:51Z","timestamp":1655914671000},"source":"Crossref","is-referenced-by-count":3,"title":["A dopamine antagonist, domperidone enhances the replication of an oncolytic adenovirus in human tumour cells"],"prefix":"10.1099","volume":"103","author":[{"given":"Fumitaka","family":"Nishimae","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1366-9662","authenticated-orcid":false,"given":"Fuminori","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka 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