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In conventional NDV-based vectors, the transgene is arranged as a separate transcription unit in the NDV genome. Here, we expressed haemagglutinin protein (HA) of an avian influenza virus using an NDV vector design in which the transgene ORF is encoded in-frame with the ORF of an NDV gene. This arrangement does not increase the number of transcription units in the NDV genome, and imposes a selection pressure against mutations interrupting the transgene ORF. We placed the HA ORF upstream or downstream of N, M, F and HN ORFs of NDV so that both proteins are encoded in-frame and are separated by either a self-cleaving 2A peptide, furin cleavage site or both. Only constructs in which HA was placed downstream of the NDV HN were viable. These constructs expressed the transgene at a higher level compared to the vector encoding the same transgene in the same position in the NDV genome but as a separate transcription unit. Furthermore, the transgene expressed in one ORF with the NDV protein proved to be more stable over multiple passages. Thus, this design may be useful for applications where the stability of the transgene expression is highly important for a recombinant NDV vector.<\/jats:p>","DOI":"10.1099\/jgv.0.001761","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T14:17:22Z","timestamp":1656339442000},"source":"Crossref","is-referenced-by-count":4,"title":["Encoding of a transgene in-frame with a Newcastle disease virus protein increases transgene expression and stability"],"prefix":"10.1099","volume":"103","author":[{"given":"Mohamed A.","family":"Elbehairy","sequence":"first","affiliation":[{"name":"Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt"},{"name":"Virginia-Maryland College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, MD 20740, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siba K.","family":"Samal","sequence":"additional","affiliation":[{"name":"Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt"},{"name":"Virginia-Maryland College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, MD 20740, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0892-1731","authenticated-orcid":false,"given":"George A.","family":"Belov","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, MD 20740, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","first-page":"576","volume-title":"Newcastle disease, chapter 2.3.14. 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