{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T22:22:25Z","timestamp":1793226145502,"version":"4.5.0"},"reference-count":42,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T00:00:00Z","timestamp":1689120000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,12]]},"abstract":"<jats:p>Mosquito-borne dengue disease is caused by the dengue virus serotype-1 to serotype-4. The contemporary dengue outbreaks in the southwestern Indian ocean coincided with the widespread of dengue virus serotype 2 genotype II (Cosmopolitan), including epidemic viral strains DES-14 and RUN-18 isolated in Dar es Salaam (Tanzania) in 2014 and La Reunion Island (France) in 2018, respectively. Heterodimeric interaction between prM (intracellular precursor of surface structural M protein) and envelope E proteins is required during the initial stage of dengue virus assembly. Amino acid 127 of DES-14 prM protein (equivalent to M36) has been identified as an infrequent valine whereas RUN-18 has a common isoleucine. In the present study, we examined the effect of M-I36V mutation on the expression of a recombinant RUN-18 E protein co-expressed with prM in human epithelial A549 cells. The M ectodomain of dengue virus serotype 2 embeds a pro-apoptotic peptide referred as D<jats:sub>2<\/jats:sub>AMP. The impact of M-I36V mutation on the death-promoting capability of D<jats:sub>2<\/jats:sub>AMP was assessed in A549 cells. We showed that valine at position M36 affects expression of recombinant RUN-18 E protein and potentiates apoptosis-inducing activity of D<jats:sub>2<\/jats:sub>AMP. We propose that the nature of M residue 36 influences the virological characteristics of dengue 2 M and E proteins belonging to genotype II that contributes to global dengue burden.<\/jats:p>","DOI":"10.1099\/jgv.0.001872","type":"journal-article","created":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T15:16:27Z","timestamp":1689174987000},"source":"Crossref","is-referenced-by-count":3,"title":["Dengue virus M and E proteins belonging to genotype II (Cosmopolitan) of serotype 2 are influenced by the nature of M residue 36"],"prefix":"10.1099","volume":"104","author":[{"given":"Jason","family":"Decotter","sequence":"first","affiliation":[{"name":"Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Universit\u00e9 de La R\u00e9union, INSERM U1187, CNRS 9192, IRD 249, Plateforme Technologique CYROI, 97490 Sainte-Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-4050","authenticated-orcid":false,"given":"Philippe","family":"Despr\u00e8s","sequence":"additional","affiliation":[{"name":"Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Universit\u00e9 de La R\u00e9union, INSERM U1187, CNRS 9192, IRD 249, Plateforme Technologique CYROI, 97490 Sainte-Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Gadea","sequence":"additional","affiliation":[{"name":"Present address: MetaSarc team Inserm U1194, Institut de Recherche en Canc\u00e9rologie de Montpellier (IRCM), F-34298 Montpellier, France"},{"name":"Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Universit\u00e9 de La R\u00e9union, INSERM U1187, CNRS 9192, IRD 249, Plateforme Technologique CYROI, 97490 Sainte-Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0476-8"},{"key":"R3","article-title":"Dengue and Severe Dengue","year":"2022"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(14)60016-X"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0357-z"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.12.001"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4268-4275.2001"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1067"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-74-2-175"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1126\/science.1153263"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/nsb990"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.67.3.1672-1675.1993"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.76.11.5480-5491.2002"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.71.11.8475-8481.1997"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2463"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19163-0"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1016\/s0300-9084(03)00139-1"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01176-15"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00086-20"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.11.006"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-92783-y"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.3201\/eid2808.220550"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e01455"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01443-19"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.3201\/eid2205.151462"},{"key":"R26","article-title":"Surveillance de la dengue \u00e0 La R\u00e9union","year":"2023"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010547"},{"key":"R28","article-title":"Situation de La Dengue \u00e0 La R\u00e9union","year":"2019"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041951"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00937-14"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249578"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.610428"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.3390\/v9090243"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031287"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.654494"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1002\/cm.10057"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.03.028"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-02862-7"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00996-19"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.ac2019.po0333"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4877"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.068023-0"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001872?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T15:16:29Z","timestamp":1689174989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.001872"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,12]]},"references-count":42,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7,12]]}},"URL":"https:\/\/doi.org\/10.1099\/jgv.0.001872","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,12]]}}}