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In this study, rodents (<jats:italic>n<\/jats:italic>=91) from seven different species and ten pikas (<jats:italic>Ochotona curzoniae<\/jats:italic>) were collected in Qinghai Province, China. Using transcriptomic sequencing and confirmatory molecular testing, hepeviruses were detected in 27 of 45 (60\u200a%) long-tailed dwarf hamsters (<jats:italic>Cricetulus longicaudatus<\/jats:italic>) and were undetected in other rodents and pika. The complete genome sequences from 14 representative strains were subsequently obtained, and phylogenetic analyses suggested that they represent a novel species within the genus <jats:italic>Rocahepevirus<\/jats:italic>, which we tentatively designated as Cl-2018QH. The virus was successfully isolated in human hepatoma (Huh-7) and murine fibroblast (17\u2009Cl-1) cell lines, though both exhibited limited replication as assayed by detection of negative-sense RNA intermediates. A129 immunodeficient mice were inoculated with Cl-2018QH and the virus was consistently detected in multiple organs, despite relatively low viral loads. In summary, this study has described a novel rodent hepevirus, which enhances our knowledge of the genetic diversity of rodent hepeviruses and highlights its potential for cross-species transmission.<\/jats:p>","DOI":"10.1099\/jgv.0.001989","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T15:16:14Z","timestamp":1716218174000},"source":"Crossref","is-referenced-by-count":0,"title":["Isolation and characterization of a novel rodent hepevirus in long-tailed dwarf hamsters (Cricetulus longicaudatus) in China"],"prefix":"10.1099","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5322-1435","authenticated-orcid":false,"given":"Lin","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, PR China"},{"name":"School of Public Health, Shandong First Medical University & Shandong 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