{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:46Z","timestamp":1792786186071,"version":"4.5.0"},"reference-count":57,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft","award":["497659464"],"award-info":[{"award-number":["497659464"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Deutsche Forschungsgemeinschaft","award":["BE 5748\/1-2, STE 1428\/5-2, 398066876\/GRK 2485\/1"],"award-info":[{"award-number":["BE 5748\/1-2, STE 1428\/5-2, 398066876\/GRK 2485\/1"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,28]]},"abstract":"<jats:p>\n                    Mosquitoes are known to transmit different arthropod-borne viruses belonging to various virus families. The exogenous small interfering RNA pathway plays an important role in the mosquito defence against such virus infections, with Dicer-2 (Dcr2) as one of the key proteins that initiates the cleavage of viral dsRNAs into 21 nt long virus-derived small interfering RNAs. Previous data identified the importance of various motifs in Dcr2 for its small interfering RNA (siRNA)-mediated antiviral activity. However, all these data focus on positive-strand RNA viruses, although negative-strand RNA viruses, like\n                    <jats:italic>Bunyaviricetes<\/jats:italic>\n                    , include several important mosquito-borne viruses. Here, we aim to investigate the importance of different domains of Dcr2 for antiviral activity against viruses of the\n                    <jats:italic>Bunyaviricetes<\/jats:italic>\n                    . For this, we used the\n                    <jats:italic>Aedes aegypti-<\/jats:italic>\n                    derived Dcr2 knock-out cell line Aag2-AF319 to study the importance of the helicase, RNase III and PIWI\u2013Argonaute\u2013Zwille domains of Dcr2 on the antiviral activity of two viruses belonging to different families of the\n                    <jats:italic>Bunyaviricetes<\/jats:italic>\n                    : the Rift Valley fever virus (RVFV) vaccine strain MP12 (\n                    <jats:italic>Phenuiviridae<\/jats:italic>\n                    ,\n                    <jats:italic>Phlebovirus<\/jats:italic>\n                    ) and the Bunyamwera orthobunyavirus (BUNV;\n                    <jats:italic>Peribunyaviridae<\/jats:italic>\n                    ,\n                    <jats:italic>Orthobunyavirus<\/jats:italic>\n                    ). All three domains were determined to be critical for the antiviral activity against both RVFV and BUNV. Interestingly, one specific mutation in the helicase domain (KN) did not result in a loss of antiviral activity for RVFV, but for BUNV, despite losing the ability to produce 21 nt siRNAs.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.002046","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T10:16:22Z","timestamp":1730974582000},"source":"Crossref","is-referenced-by-count":1,"title":["Dicer-2 mutations in Aedes aegypti cells lead to a diminished antiviral function against Rift Valley fever virus and Bunyamwera virus infection"],"prefix":"10.1099","volume":"105","author":[{"given":"Susann","family":"Dornbusch","sequence":"first","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Hannover, Germany"},{"name":"Institute for Parasitology and Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Hannover, 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