{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T10:54:06Z","timestamp":1753181646556,"version":"3.32.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012891","name":"Medical Research Charities Group","doi-asserted-by":"crossref","award":["IAA 2022-2026"],"award-info":[{"award-number":["IAA 2022-2026"]}],"id":[{"id":"10.13039\/100012891","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,10]]},"abstract":"<jats:p>Parainfluenza virus type 5 (PIV5) can cause either persistent or acute\/lytic infections in a wide range of mammalian tissue culture cells. Here, we have generated PIV5 fusion (F)-expressing helper cell lines that support the replication of F-deleted viruses. As proof of the principle that F-deleted single-cycle infectious viruses can be used as safe and efficient expression vectors, we have cloned and expressed a humanized (Hu) version of the mouse anti-V5 tag antibody (clone SV5-Pk1). We show that multiple different cell lines can be infected and express high levels of the Hu anti-V5 antibody, with Chinese hamster ovary cells expressing 20\u201350\u2009mg l<jats:sup>\u22121<\/jats:sup> after 5\u2009days when cells were grown to a density of ~1\u00d710<jats:sup>6<\/jats:sup>\u2009cells\u2009per millilitre at the time of infection. We suggest that PIV5-based vectors may be further developed to produce recombinant proteins both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jgv.0.002061","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T15:17:17Z","timestamp":1736435837000},"source":"Crossref","is-referenced-by-count":1,"title":["Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody"],"prefix":"10.1099","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9304-6678","authenticated-orcid":false,"given":"Richard E.","family":"Randall","sequence":"first","affiliation":[{"name":"School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Young","sequence":"additional","affiliation":[{"name":"School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Pisliakova","sequence":"additional","affiliation":[{"name":"School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jelena","family":"Andrejeva","sequence":"additional","affiliation":[{"name":"School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynsey","family":"West","sequence":"additional","affiliation":[{"name":"School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Rossler","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Clinic rechts der Isar, School of Medicine, Technical University of Munich, 81675 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"Morath","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Clinic rechts der Isar, School of Medicine, Technical University of Munich, 81675 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Hughes","sequence":"additional","affiliation":[{"name":"School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St. Andrews, St. Andrews, Fife, KY16 9ST, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Goodbourn","sequence":"additional","affiliation":[{"name":"Section for Pathogen Research, Institute for Infection and Immunity, St George's, University of London, London, SW17 0RE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2023.102572"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03351-13"},{"key":"R3","first-page":"241","article-title":"Parainfluenza-5 virus. 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