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Studying the interaction patterns between SADS-CoV and host proteins can provide guidance for the development of antiviral drugs. In previous work, we identified 289 host proteins interacting with the SADS-CoV M protein through glutathione S-transferase pull down combined with LC-MS\/MS. Here, we focus on prohibitin (PHB), which is associated with the stability of mitochondrial function in cells, and demonstrate that there is a physical interaction and cellular co-localization relationship between the SADS-CoV M protein and PHB protein. Additionally, SADS-CoV-mediated infection has a strong correlation with PHB expression, and regulating PHB expression dose-dependently antagonizes SADS-CoV replication. Moreover, we discovered that PHB has an antagonistic effect on apoptosis induced by SADS-CoV infection. Overall, this work helps to elucidate the role of the PHB protein in the SADS-CoV life cycle, providing a potential target for antiviral research.<\/jats:p>","DOI":"10.1099\/jgv.0.002073","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T15:54:56Z","timestamp":1739289296000},"source":"Crossref","is-referenced-by-count":0,"title":["Prohibitin of swine antagonizes SADS-CoV replication and virus-induced apoptosis"],"prefix":"10.1099","volume":"106","author":[{"given":"Jingya","family":"Xu","sequence":"first","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou, PR 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