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During a 2021\u20132022 rabies virus (RABV) outbreak in South Africa, we collected cervical lymph nodes from 36 rabies-suspect dogs; RABV RNA was detected in 27. Canine distemper virus RNA was detected in a subset across both RABV-positive (RABV+) and RABV-negative (RABV\u2212) groups and was not associated with clinical-sign count. We set up a computer-assisted histological analysis tool to quantify germinal-centre (GC) nucleus density and immunohistochemistry for CD20, PNA and IBA1 to profile B cells, GC activity and macrophages. Within the outbreak cohort, GC density and marker-based metrics did not differ between RABV+ and RABV\u2212 dogs. Two healthy dogs were included as reference tissues; values in outbreak dogs were generally lower, but these contrasts are contextual given the limited, non-matched controls. 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