{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T13:48:25Z","timestamp":1780926505138,"version":"3.54.1"},"reference-count":74,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yomogi Inc","award":["2025"],"award-info":[{"award-number":["2025"]}]},{"name":"JSPS","award":["21H05035"],"award-info":[{"award-number":["21H05035"]}]},{"name":"JSPS","award":["25K21734"],"award-info":[{"award-number":["25K21734"]}]},{"name":"JSPS","award":["23H02214"],"award-info":[{"award-number":["23H02214"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,17]]},"abstract":"<jats:p>\n                    A new curvulavirid was isolated from a Japanese strain of the filamentous ascomycete Cryphonectria nitschkei and thoroughly characterized. The virus termed Cryphonectria nitschkei curvulavirus 1 (CnCvV1) has a bi-segmented dsRNA genome. CnCvV1 dsRNA1 encodes an RNA-dependent RNA polymerase (592 amino acids), while dsRNA2 possesses two ORFs, one that encodes a protein associated with the genomic dsRNA and the other that encodes a hypothetical protein of unknown function. CnCvV1 could be experimentally introduced into another virus-free strain of\n                    <jats:italic>C. nitschkei<\/jats:italic>\n                    and two strains of different fungal species within the genus\n                    <jats:italic>Cryphonectria<\/jats:italic>\n                    (\n                    <jats:italic>Cryphonectria parasitica<\/jats:italic>\n                    and\n                    <jats:italic>Cryphonectria carpinicola<\/jats:italic>\n                    ). Based on phenotypic comparison, the virus caused asymptomatic infection in the three newly established fungal strains. However, there was a reduced colony growth rate and increased CnCvV1 accumulation in an RNA silencing-deficient mutant (\u0394\n                    <jats:italic>dcl2<\/jats:italic>\n                    ), relative to the wt strain EP155 of a model virus host fungus (\n                    <jats:italic>C. parasitica<\/jats:italic>\n                    ). These findings suggest that CnCvV1 is targeted by RNA silencing in\n                    <jats:italic>C. parasitica<\/jats:italic>\n                    . This study provides a foundation for further exploration of curvulavirids that have been biologically understudied.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.002177","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T17:16:18Z","timestamp":1765991778000},"source":"Crossref","is-referenced-by-count":3,"title":["Thorough characterization of a new curvulavirid from a Japanese strain of Cryphonectria nitschkei"],"prefix":"10.1099","volume":"106","author":[{"given":"Sabitree","family":"Shahi","sequence":"first","affiliation":[{"name":"Present address: Immunology Section, Liver Diseases Branch, NIDDK, National Institutes of Health, DHHS, Bethesda, MD 20892, USA"},{"name":"Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakae","family":"Hisano","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, 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