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It has been recently shown that \u03b3\u03b4 T cells restrict Marek\u2019s disease virus (MDV) pathogenesis; however, it remained elusive if they play a role in vaccine protection. In this study, we vaccinated \u03b3\u03b4 T-cell-knockout chickens with the commercial turkey herpesvirus (HVT) vaccine and challenged them with very virulent MDV. The disease incidence was significantly increased in vaccinated chickens in the absence of \u03b3\u03b4 T cells. This increase was comparable to a previous study in unvaccinated \u03b3\u03b4 T-cell-knockout chickens, suggesting that \u03b3\u03b4 T cells only play a minor role in vaccine protection. Furthermore, the viral load in the spleen was significantly increased in the absence of \u03b3\u03b4 T cells. Interestingly, viral load in the skin and in dust shed by the animals was drastically increased, suggesting that the absence of \u03b3\u03b4 T cells affects MDV shedding. In addition, we quantified various immune cell subsets to determine if these could be responsible for the observed phenotypes. Together, our data indicate that \u03b3\u03b4 T cells only play a minor role in HVT-mediated protection, but their absence drastically affects shedding of this deadly pathogen in vaccinated animals.<\/jats:p>","DOI":"10.1099\/jgv.0.002204","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T16:17:12Z","timestamp":1769789832000},"source":"Crossref","is-referenced-by-count":0,"title":["Role of \u03b3\u03b4 T cells in turkey herpesvirus vaccine protection against Marek\u2019s disease virus"],"prefix":"10.1099","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2266-0128","authenticated-orcid":false,"given":"Mohammad A.","family":"Sabsabi","sequence":"first","affiliation":[{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin, Berlin 14163, Germany"},{"name":"Institut f\u00fcr Virologie, Freie Universit\u00e4t Berlin, Berlin 14163, 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