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In this study, we characterized two Chinook salmon embryo-derived cell lines \u2013 a wild-type and a Protein Kinase R (PKR)-deficient cell line \u2013 which were found to be persistently infected with IPNV. Both cell lines displayed hallmark features of viral persistence, including sustained cell growth without cytopathic effect, continuous production of infectious virus over successive passages and resistance to superinfection by homologous IPNV strains and susceptibility to heterologous viruses. Longitudinal analyses over 40 weekly passages revealed pronounced oscillations in extracellular infectious titres, which partially correlated with fluctuations in intracellular viral RNA levels. Despite these variations in viral replication, persistent infection did not trigger a robust type I interferon response, and basal expression of key interferon-stimulated genes was dampened. Notably, the absence of PKR did not alter viral persistence dynamics, indicating that PKR is not a regulator of this process. Taken together, these findings suggest that persistent IPNV employs immune evasion strategies to suppress host innate immune signalling while maintaining low-level replication.<\/jats:p>","DOI":"10.1099\/jgv.0.002283","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:16:47Z","timestamp":1781799407000},"source":"Crossref","is-referenced-by-count":1,"title":["Characterization of cell lines persistently infected with infectious pancreatic necrosis virus"],"prefix":"10.1099","volume":"107","author":[{"given":"Lise","family":"Chaumont","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Peruzzi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Huetz","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222, Universit\u00e9 de Paris, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Raffy","sequence":"additional","affiliation":[{"name":"VIRBAC S.A., Carros, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Le Hir","sequence":"additional","affiliation":[{"name":"VIRBAC S.A., Carros, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jules","family":"Minke","sequence":"additional","affiliation":[{"name":"VIRBAC S.A., Carros, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Boudinot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2795-2653","authenticated-orcid":false,"given":"Bertrand","family":"Collet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","first-page":"26","article-title":"Infectious pancreatic necrosis in brook trout","volume":"60","author":"Wood","year":"1955","journal-title":"AMA Arch Pathol"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701048200"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012374410-4.00772-X"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.01.043"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2006.04.009"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(94)00392-2"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9020094"},{"key":"R8","doi-asserted-by":"crossref","DOI":"10.1079\/9781845935542.0001","volume-title":"Infectious Pancreatic Necrosis and Associated Aquatic Birnaviruses. 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