{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:04:50Z","timestamp":1784307890893,"version":"3.55.0"},"reference-count":67,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Research Council","award":["MR\/W007002\/1"],"award-info":[{"award-number":["MR\/W007002\/1"]}]},{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"crossref","award":["NE\/S00713X\/1"],"award-info":[{"award-number":["NE\/S00713X\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/T007222\/1"],"award-info":[{"award-number":["BB\/T007222\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/W013800\/1"],"award-info":[{"award-number":["BB\/W013800\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:p>\n                    Antimicrobial resistance poses a critical global health threat, with enterococci among the leading contributors due to their intrinsic and acquired resistance to antibiotics. Clinically relevant species, including\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    and\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    as well as the emerging poultry pathogen\n                    <jats:italic>Enterococcus cecorum<\/jats:italic>\n                    , highlight the need for alternative therapeutics across human and agricultural settings. Bacteriophages and their derived enzymes, particularly endolysins, offer promising antibacterial strategies but challenges such as phage resistance and limited lysin diversity hinder their application. In this study, we performed a large-scale analysis of prophage-encoded endolysins across these three enterococcal opportunistic pathogens, characterizing over 48,000 sequences. We identified 33 distinct domain architectures combining diverse catalytic and cell wall-binding domains (CBDs) including novel putative CBDs. These findings expand the known diversity of enterococcal lysins and provide a comprehensive resource for the rational design of stable, recombinant \u2018enzybiotics\u2019 to combat multidrug-resistant enterococcal infections.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.002292","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:16:33Z","timestamp":1784304993000},"source":"Crossref","is-referenced-by-count":0,"title":["Data mining reveals the diversity of prophage endolysins targeting pathogenic enterococci"],"prefix":"10.1099","volume":"107","author":[{"given":"Finn","family":"O'Dea","sequence":"first","affiliation":[{"name":"The Florey Institute of Infection, University of Sheffield, Sheffield, UK"},{"name":"Molecular Microbiology, School of Biosciences, University of Sheffield, Sheffield, 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