{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:59:47Z","timestamp":1786550387166,"version":"build-2736575974"},"reference-count":34,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["U19 AI181984"],"award-info":[{"award-number":["U19 AI181984"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["UC7 AI180311"],"award-info":[{"award-number":["UC7 AI180311"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI161765"],"award-info":[{"award-number":["R01 AI161765"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI169850"],"award-info":[{"award-number":["R01 AI169850"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI150792"],"award-info":[{"award-number":["R01 AI150792"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["K01 AI165965"],"award-info":[{"award-number":["K01 AI165965"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["T32 AI049820"],"award-info":[{"award-number":["T32 AI049820"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["F32 AI191453"],"award-info":[{"award-number":["F32 AI191453"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,12]]},"abstract":"<jats:p>Rift Valley fever virus (RVFV) is a mosquito-borne phlebovirus that poses a growing global threat, causing severe disease in humans and livestock, including hepatitis, haemorrhage and encephalitis. Documented cases of vertical transmission in humans and livestock highlight risks during pregnancy. Although RVFV can directly infect the placenta, how it enters trophoblast cells remains unclear. Here, we investigated the role of low-density lipoprotein receptor-related protein 1 (LRP1), a known RVFV entry factor, in human trophoblast infection. Using JEG-3, JAR and HTR-8\/SVneo trophoblast cell lines, we demonstrate that RVFV replicates to high titres and LRP1 expression varies across cell types. Competitive inhibition assays using the high-affinity LRP1 ligand murine RAP domain 3 and recombinant LRP1 fragments (cluster proteins) indicate that RVFV requires LRP1 for efficient trophoblast infection. Together, these findings implicate LRP1 as a determinant of RVFV infection at the maternal-fetal interface.<\/jats:p>","DOI":"10.1099\/jgv.0.002300","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:25:42Z","timestamp":1786548342000},"source":"Crossref","is-referenced-by-count":0,"title":["Rift Valley fever virus infects trophoblast cell lines in an LRP1-dependent manner"],"prefix":"10.1099","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7481-7134","authenticated-orcid":false,"given":"Rachael E.","family":"Rush","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA"},{"name":"Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9211-7908","authenticated-orcid":false,"given":"Cynthia M.","family":"McMillen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA"},{"name":"Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Donermeyer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7189-9557","authenticated-orcid":false,"given":"Daisy W.","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA"},{"name":"Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0418-9707","authenticated-orcid":false,"given":"Gaya K.","family":"Amarasinghe","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0857-2973","authenticated-orcid":false,"given":"Amy L.","family":"Hartman","sequence":"additional","affiliation":[{"name":"Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA"},{"name":"Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202302005"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.09.001"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2023.09.001"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01841-25"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04326-0"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2204706119"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1017\/S204017441900076X"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(79)90004-x"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.20506\/rst.18.3.1195"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01394-19"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1017\/s0950268800068011"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1179\/146532806X120363"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21132"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(80)90167-4"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(16)30176-0"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00617-0"},{"key":"R17","volume-title":"Epidemiological Alert Oropouche in the Region of the Americas: Vertical Transmission Event under Investigation in Brazil","year":"2024"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.002027"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aat6114"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00610-y"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00983-24"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2016.12.007"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.5115\/acb.20.234"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2022.106138"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2019.12.013"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2004.06.038"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/s0962-8924(98)01283-5"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1016\/s0074-7696(01)09011-8"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.3390\/biom16010014"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abh2169"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-025-03616-4"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.660084"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.05.009"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau9812"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.002300?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:25:47Z","timestamp":1786548347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/jgv.0.002300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,12]]}},"URL":"https:\/\/doi.org\/10.1099\/jgv.0.002300","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]}}}