{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:29Z","timestamp":1792786169860,"version":"4.5.0"},"reference-count":54,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sigrid Jus\u00e9liuksen S\u00e4\u00e4ti\u00f6","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"https:\/\/ror.org\/00ckakm23","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"https:\/\/ror.org\/03vxy9y38","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:p>\n                    Hepatitis D virus (HDV) is a satellite virus that utilizes hepatitis B virus (HBV) as a helper for infectious particle formation. HDV was originally identified as a novel antigen in liver biopsies of HBV patients, and later studies showed the \u2018delta\u2019 antigen (DAg) to be the sole protein encoded by HDV. Until the discovery of HDV-\n                    <jats:italic>like<\/jats:italic>\n                    agents in birds and snakes in 2018, HDV was a unique example of animal satellite viruses. We identified Swiss snake colony virus 1 (SwSCV-1) in the brain of a\n                    <jats:italic>Boa constrictor<\/jats:italic>\n                    and through comparison we found the genome organization of SwSCV-1 to resemble that of HDV. However, in addition to the DAg ORF, the genome of SwSCV-1 includes another &gt;500\u2009nt ORF, \u2018ORF2\u2019. To study whether the putative ORF2-encoded protein plays a role in the SwSCV-1 life cycle, we established an infectious clone of the virus with a point mutation in the methionine initiation codon of ORF2. The mutation did not significantly affect initiation of replication, establishment of persistent infection or infectious particle formation upon superinfection with a helper virus. Using additional methods, we gathered further evidence confirming that ORF2 is not actively translated in boa constrictor cells. We further showed that unlike HDV, SwSCV-1 expresses a single form of the DAg. Although the proteins encoded by SwSCV-1 and HDV only include one and two forms of the DAg, respectively, whether other kolmioviruses express additional forms of DAg or related proteins in some cell types or host species merits further research.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.002327","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:17:09Z","timestamp":1791386229000},"source":"Crossref","is-referenced-by-count":0,"title":["Snake kolmiovirus encodes a single form of delta antigen and shows no evidence of translation from ORF2"],"prefix":"10.1099","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1528-044X","authenticated-orcid":false,"given":"Leon\u00f3ra","family":"Szirovicza","sequence":"first","affiliation":[{"name":"Medicum, Department of Virology, University of Helsinki, Helsinki, 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