{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:13:22Z","timestamp":1788455602210,"version":"build-2803163510"},"reference-count":29,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Academic Exchange Service","award":["57818587"],"award-info":[{"award-number":["57818587"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,3]]},"abstract":"<jats:p>\n                    High-throughput sequencing (HTS) technologies have transformed life sciences by enabling rapid, large-scale analysis of nucleic acids, providing unprecedented insights into genomics and transcriptomics. Accurate determination of RNA termini remains, however, a major challenge. We have addressed this limitation by fine-tuning a commercial HTS library preparation protocol. The optimized strategy was validated on plant virus isolates representing diverse taxonomic groups and genome architectures and benchmarked against conventional RACE, demonstrating its effectiveness and robustness. Investigations in members of the genus\n                    <jats:italic>Ilarvirus<\/jats:italic>\n                    allowed to prove the occurrence of non-templated nucleotide additions at the 3\u2032 end of the minus-strand viral RNAs\n                    <jats:italic>in vivo<\/jats:italic>\n                    , expanding previous results obtained\n                    <jats:italic>in vitro<\/jats:italic>\n                    in related members in the\n                    <jats:italic>Bromoviridae<\/jats:italic>\n                    and supporting that this feature may be common across the family.\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.002331","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T16:15:30Z","timestamp":1788452130000},"source":"Crossref","is-referenced-by-count":0,"title":["Enhanced recovery of terminal sequences of viral RNAs via fine-tuning of the high-throughput sequencing library preparation and evidence of a non-templated nucleotide addition at the 3\u2032 end of the minus strand in members of the genus Ilarvirus"],"prefix":"10.1099","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3614-8635","authenticated-orcid":false,"given":"Dennis","family":"Knierim","sequence":"first","affiliation":[{"name":"Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstrasse 7B, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8670-1331","authenticated-orcid":false,"given":"Paolo","family":"Margaria","sequence":"additional","affiliation":[{"name":"Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstrasse 7B, 38124 Braunschweig, 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