{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:47:18Z","timestamp":1791996438676,"version":"4.4.0"},"reference-count":42,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"G\u00e9nome Qu\u00e9bec","id":[{"id":"https:\/\/ror.org\/00xhtr962","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:p>\n                    RNA interference (RNAi) is a central antiviral defence mechanism in fungi, yet relatively few mycoviral suppressors of RNA silencing (VSRs) have been functionally characterized, particularly in phytopathogenic hosts. Botrytis virus X (BVX), a positive-sense RNA virus in the family\n                    <jats:italic>Alphaflexiviridae<\/jats:italic>\n                    , infects\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    and encodes five predicted ORFs, most of which have unknown functions. Here, we screened BVX ORFs 2\u20135 for RNA silencing suppressor activity using complementary GFP-based assays in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    and examined the leading candidate in the fungal host\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    . BVX ORF2 (X2) enhanced GFP transcript and protein accumulation in assays where silencing was triggered by sense RNA but failed to suppress silencing triggered by hairpin-derived small interfering RNAs or microRNA-guided targeting, indicating a trigger-restricted suppressor phenotype. In\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    , transgenic expression of X2 was associated with reduced induction of the RNAi-associated genes\n                    <jats:italic>BcDCL1<\/jats:italic>\n                    and\n                    <jats:italic>BcDCL2<\/jats:italic>\n                    compared with empty-vector controls, with the strongest effect observed on\n                    <jats:italic>BcDCL1<\/jats:italic>\n                    . In a virus-infected fungal background, X2 expression was also associated with increased viral RNA accumulation. Together, these results identify BVX X2 as a BVX-encoded, trigger-restricted suppressor of RNA silencing and link its expression to altered RNAi-related gene induction and increased viral RNA accumulation in\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1099\/jgv.0.002343","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:15:59Z","timestamp":1791994559000},"source":"Crossref","is-referenced-by-count":0,"title":["Botrytis virus X ORF2 suppresses RNA silencing in a trigger-restricted manner and is associated with altered Dicer-like gene expression in Botrytis cinerea"],"prefix":"10.1099","volume":"107","author":[{"given":"Fayruza","family":"Lalany","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biologie, Universit\u00e9 de Sherbrooke, Sherbrooke, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C.","family":"Drury","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Saint-Jean-sur-Richelieu, QC, Canada"},{"name":"D\u00e9partement de Biologie, Universit\u00e9 de Sherbrooke, Sherbrooke, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou L.","family":"Fall","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Saint-Jean-sur-Richelieu, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0678-4531","authenticated-orcid":false,"given":"Peter","family":"Moffett","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie, Universit\u00e9 de Sherbrooke, Sherbrooke, QC, 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