{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:54:07Z","timestamp":1790506447947,"version":"4.1.0"},"reference-count":29,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,5,1]]},"abstract":"<jats:p>\n                  <jats:bold>Purpose.<\/jats:bold> Non-host-adapted <jats:italic>Salmonella<\/jats:italic> serovars, including the common human food-borne pathogen <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium (<jats:italic>S.<\/jats:italic> Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against <jats:italic>Salmonella<\/jats:italic> are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> An attenuated <jats:italic>S<\/jats:italic>. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent <jats:italic>S.<\/jats:italic> Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNA-Seq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Vaccination significantly reduced fever and interferon-gamma (IFN\u03b3) levels in swine challenged with virulent <jats:italic>S<\/jats:italic>. Typhimurium compared to mock-vaccinated pigs. <jats:italic>Salmonella<\/jats:italic> faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental <jats:italic>S.<\/jats:italic> Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcine-derived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Evaluation of the <jats:italic>S<\/jats:italic>. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.<\/jats:p>","DOI":"10.1099\/jmm.0.000482","type":"journal-article","created":{"date-parts":[[2017,5,18]],"date-time":"2017-05-18T11:12:22Z","timestamp":1495105942000},"page":"651-661","source":"Crossref","is-referenced-by-count":21,"title":["Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine"],"prefix":"10.1099","volume":"66","author":[{"given":"Bradley L","family":"Bearson","sequence":"first","affiliation":[{"name":"USDA\/ARS\/National Laboratory for Agriculture and the Environment, Ames, IA, 50011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn M. D","family":"Bearson","sequence":"additional","affiliation":[{"name":"USDA\/ARS\/National Animal Disease Center, Ames, IA, 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian W","family":"Brunelle","sequence":"additional","affiliation":[{"name":"USDA\/ARS\/National Animal Disease Center, Ames, IA, 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darrell O","family":"Bayles","sequence":"additional","affiliation":[{"name":"USDA\/ARS\/National Animal Disease Center, Ames, IA, 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In Soo","family":"Lee","sequence":"additional","affiliation":[{"name":"Hannam University, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalusa D","family":"Kich","sequence":"additional","affiliation":[{"name":"Embrapa Swine and Poultry, Conc\u00f3rdia, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.12.017"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12137"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2014.00009"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12256"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.01.036"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2010.03.005"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2016.00066"},{"key":"R8","volume-title":"FastQC: A Quality Control Tool for High Throughput Sequence Data","author":"Andrews","year":"2010"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043592"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00801"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-954"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-3-r25"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"R17","volume-title":"topGO: Enrichment Analysis for Gene Ontology","author":"Alexa","year":"2016"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2007.10.001"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.06.008"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.074"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1177\/1753425914525812"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1111\/risa.12586"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6446-6452.2003"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1159\/000274309"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06505.x"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028768"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2011.06.001"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-83"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00825-07"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.000482?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T11:32:51Z","timestamp":1702639971000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.000482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,1]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.000482","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,1]]}}}