{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:49:37Z","timestamp":1787701777050,"version":"build-2784847793"},"reference-count":9,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:p>The macrolide azithromycin is recommended for treatment of <jats:italic>Mycoplasma genitalium<\/jats:italic> infection; however, <jats:italic>M. genitalium<\/jats:italic> strains possessing macrolide resistance-mediating mutations (MRMMs) are increasingly being reported. Here, we used the SpeeDx ResistancePlus MG kit, which provides simultaneous detection of <jats:italic>M. genitalium<\/jats:italic> and MRMMs, to assess MRMM carriage among <jats:italic>M. genitalium<\/jats:italic> infections in Queensland, Australia. Performance characteristics of the ResistancePlus MG kit for <jats:italic>M. genitalium<\/jats:italic> detection were compared to in-house PCR. Available <jats:italic>M. genitalium<\/jats:italic> PCR-positive (<jats:italic>n<\/jats:italic>=67) and negative (<jats:italic>n<\/jats:italic>=281) samples from the years 2011 to 2017 were tested using the SpeeDx ResistancePlus MG kit. In total, 63.6\u200a% <jats:italic>M. genitalium<\/jats:italic>-positive samples were indicated to harbour MRMMs. The ResistancePlus MG method provided sensitivity and specificity of 97 and 99.6\u200a%\u2009respectively compared to in-house PCR for <jats:italic>M. genitalium<\/jats:italic> detection. Such high levels of macrolide-resistant <jats:italic>M. genitalium<\/jats:italic> raise further concerns over future use of azithromycin for treatment of <jats:italic>M. genitalium<\/jats:italic> infection.<\/jats:p>","DOI":"10.1099\/jmm.0.000584","type":"journal-article","created":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T09:12:49Z","timestamp":1505207569000},"page":"1451-1453","source":"Crossref","is-referenced-by-count":24,"title":["High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia"],"prefix":"10.1099","volume":"66","author":[{"given":"Ella","family":"Trembizki","sequence":"first","affiliation":[{"name":"UQ Centre for Clinical Research, The University of Queensland, Brisbane, Queensland 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Buckley","sequence":"additional","affiliation":[{"name":"UQ Centre for Clinical Research, The University of Queensland, Brisbane, Queensland 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl","family":"Bletchly","sequence":"additional","affiliation":[{"name":"Pathology Queensland Central Laboratory, Brisbane, Queensland 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme R.","family":"Nimmo","sequence":"additional","affiliation":[{"name":"Pathology Queensland Central Laboratory, Brisbane, Queensland 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Whiley","sequence":"additional","affiliation":[{"name":"Pathology Queensland Central Laboratory, Brisbane, Queensland 4029, Australia"},{"name":"UQ Centre for Clinical Research, The University of Queensland, Brisbane, Queensland 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2009.009143"},{"key":"R3","first-page":"1","article-title":"Centers for disease c, prevention. sexually transmitted diseases treatment guidelines, 2015","volume":"64","author":"Workowski","year":"2015","journal-title":"MMWR Recomm Rep"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-052688"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000617"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00370-17"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw719"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02312-16"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.000584?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T11:31:56Z","timestamp":1702639916000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.000584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,1]]},"references-count":9,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,10,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.000584","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,1]]}}}