{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:38:12Z","timestamp":1761129492633},"reference-count":32,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,11,1]]},"abstract":"<jats:p>Melioidosis is an endemic infectious disease in Southeast Asia and northern Australia, caused by <jats:italic>Burkholderia pseudomallei<\/jats:italic>. However, the incidence rate in Malaysia is not well documented. The high mortality rate and broad range of clinical presentations require rapid and accurate diagnosis for appropriate treatment. This study compared the efficacy of in-house IgM and IgG ELISA methods using a local <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> strain. The diagnostic accuracy of the in-house IgG ELISA was better than that of the IgM ELISA: sensitivity (IgG: 84.71\u200a%, IgM: 76.14\u200a%) and specificity (IgG: 93.64\u200a%, IgM: 90.17\u200a%); positive predictive value (IgG: 86.75\u200a%, IgM: 79.76\u200a%) and negative predictive value (IgG: 92.57\u200a%, IgM: 89.66\u200a%); likelihood ratio (LR) [IgG: 13.32, IgM: 7.75 (LR+); IgG: 0.16, IgM: 0.26 (LR\u2013)], and was supported by the observation of the absorbance value in comparisons between culture and serology sampling. In-house IgG ELISA was shown to be useful as an early diagnostic tool for melioidosis.<\/jats:p>","DOI":"10.1099\/jmm.0.000611","type":"journal-article","created":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T13:12:33Z","timestamp":1508418753000},"page":"1623-1627","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of in-house IgM and IgG ELISAs for the serodiagnosis of melioidosis in Malaysia"],"prefix":"10.1099","volume":"66","author":[{"given":"Shirley Yi Fen","family":"Hii","sequence":"first","affiliation":[{"name":"Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, 50588 Jalan Pahang, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noor Azila","family":"Ali","sequence":"additional","affiliation":[{"name":"Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, 50588 Jalan Pahang, Kuala Lumpur, 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