{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T22:00:24Z","timestamp":1784757624250,"version":"3.55.0"},"reference-count":52,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,3,1]]},"abstract":"<jats:p>\n                  <jats:bold>Purpose.<\/jats:bold> Virulent genotypes of <jats:italic>Helicobacter pylori vacA s1m1\/cagA<jats:sup>+<\/jats:sup>\/babA2<jats:sup>+<\/jats:sup>\n                  <\/jats:italic> have been associated with severe gastric diseases. VacA, CagA and BabA are polymorphic proteins, and their association with the disease is allele-dependent. The aims of this work were: (i) to determine the prevalence of <jats:italic>H. pylori<\/jats:italic> by type of chronic gastritis; (ii) to describe the frequency of <jats:italic>cagA<\/jats:italic>, <jats:italic>babA2<\/jats:italic> and <jats:italic>vacA<\/jats:italic> genotypes in strains from patients with different types of chronic gastritis; (iii) to characterize the variable region of <jats:italic>cagA<\/jats:italic> alleles.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> A total of 164 patients with chronic gastritis were studied. Altogether, 50 <jats:italic>H. pylori<\/jats:italic> strains were isolated, and the status of <jats:italic>cagA<\/jats:italic>, <jats:italic>babA2<\/jats:italic> and <jats:italic>vacA<\/jats:italic> genotypes was examined by PCR. <jats:italic>cagA<\/jats:italic> EPIYA segment identification was performed using PCR and sequencing of <jats:italic>cagA<\/jats:italic> fragments of six randomly selected strains.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results\/Key findings.<\/jats:bold> The overall prevalence of <jats:italic>H. pylori<\/jats:italic> was 30.5\u200a%. Eighty percent of the isolated strains were <jats:italic>vacA s1m1<\/jats:italic>, and the <jats:italic>cagA<\/jats:italic> and <jats:italic>babA2<\/jats:italic> genes were detected in 74 and 32\u200a% of the strains, respectively. The most frequent genotypes were <jats:italic>vacA s1m1\/cagA<jats:sup>+<\/jats:sup>\/babA2<jats:sup>-<\/jats:sup>\n                  <\/jats:italic> and v<jats:italic>acA s1m1\/cagA<jats:sup>+<\/jats:sup>\/babA2<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>, with 40\u200a% (20\/50) and 28\u200a% (14\/50), respectively. In <jats:italic>cagA<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>, the most frequent EPIYA motif was -ABC (78.4\u200a%), and EPIYA-ABCC and -ABCCC motifs were found in 10.8\u200a% of the strains. A modified EPIYT-B motif was found in 66.6\u200a% of the sequenced strains.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold>\n                  <jats:italic>H. pylori<\/jats:italic> strains carrying <jats:italic>vacA s1m1<\/jats:italic>, <jats:italic>cagA<\/jats:italic>\n                  <jats:sup>+<\/jats:sup> and <jats:italic>babA2<\/jats:italic>\n                  <jats:sup>-<\/jats:sup> genotypes were the most prevalent in patients with chronic gastritis from the south of Mexico. In the <jats:italic>cagA<\/jats:italic>\n                  <jats:sup>+<\/jats:sup> strains, the EPIYA-ABC motif was the most common.<\/jats:p>","DOI":"10.1099\/jmm.0.000660","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T15:13:56Z","timestamp":1515597236000},"page":"314-324","source":"Crossref","is-referenced-by-count":18,"title":["vacA s1m1 genotype and cagA EPIYA-ABC pattern are predominant among Helicobacter pylori strains isolated from Mexican patients with chronic gastritis"],"prefix":"10.1099","volume":"67","author":[{"given":"Josefina","family":"Atrisco-Morales","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Cl\u00ednica, Facultad de Ciencias Qu\u00edmico-Biol\u00f3gicas, Universidad Aut\u00f3noma de Guerrero, Av. L\u00e1zaro C\u00e1rdenas s\/n C.U. Sur. Chilpancingo, Guerrero, C.P. 39090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica I.","family":"Mart\u00ednez-Santos","sequence":"additional","affiliation":[{"name":"CONACYT Research Fellow- Universidad Aut\u00f3noma de Guerrero, Facultad de Ciencias Qu\u00edmico-Biol\u00f3gicas, Universidad Aut\u00f3noma de Guerrero, Guerrero, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo","family":"Rom\u00e1n-Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda, Facultad de Ciencias Qu\u00edmico-Biol\u00f3gicas, Universidad Aut\u00f3noma de Guerrero, Guerrero, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Alarc\u00f3n-Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda, Facultad de Ciencias Qu\u00edmico-Biol\u00f3gicas, Universidad Aut\u00f3noma de Guerrero, Guerrero, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"De Sampedro-Reyes","sequence":"additional","affiliation":[{"name":"Hospital General \u201cDr. Raymundo Abarca Alarc\u00f3n\u201d, Chilpancingo, Guerrero, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Cruz-del Carmen","sequence":"additional","affiliation":[{"name":"Hospital General \u201cDr. Raymundo Abarca Alarc\u00f3n\u201d, Chilpancingo, Guerrero, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinorah N.","family":"Mart\u00ednez-Carrillo","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Cl\u00ednica, Facultad de Ciencias Qu\u00edmico-Biol\u00f3gicas, Universidad Aut\u00f3noma de Guerrero, Av. 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