{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T21:06:49Z","timestamp":1780434409307,"version":"3.54.1"},"reference-count":34,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,3,1]]},"abstract":"<jats:p>\n                  <jats:bold>Purpose.<\/jats:bold> To determine the predominant strains of <jats:italic>Bordetella pertussis<\/jats:italic> in Greece during 2010\u20132015.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Infants and children (<jats:italic>n<\/jats:italic>=1150) (15\u2009days to 14\u2009years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS<jats:italic>481<\/jats:italic>\/IS<jats:italic>1001<\/jats:italic> real-time PCR confirmed <jats:italic>Bordetella<\/jats:italic> spp.\/<jats:italic>B. pertussis<\/jats:italic> infection in 300 samples. A subset of samples (<jats:italic>n<\/jats:italic>=153) were analysed by multi-locus variable number tandem repeat analysis (MLVA) and (<jats:italic>n<\/jats:italic>=25) by sequence-based typing of the toxin promotor region (<jats:italic>ptxP<\/jats:italic>) on DNA extracted from clinical specimens.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results\/Key findings.<\/jats:bold> A complete MLVA profile was determined in 66 out of 153 samples; the <jats:italic>B. pertussis<\/jats:italic> MLVA type 27 (<jats:italic>n<\/jats:italic>=55) was the dominant genotype and all tested samples (<jats:italic>n<\/jats:italic>=25) expressed the <jats:italic>ptxP3<\/jats:italic> genotype. The vaccine coverage in the Greek population was 90\u200a%; however, the study population expressed complete coverage in 2 out of 264 infants (0\u201311\u2009months) and in 20 out of 36 children (1\u201314\u2009years). Roma and immigrant minorities represent 7\u200a% of the Greek population, but make up 50\u200a% of the study population, indicating a low vaccine coverage among these groups.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions.<\/jats:bold> The <jats:italic>B. pertussis<\/jats:italic> MT27 and <jats:italic>ptxP3<\/jats:italic> genotype is dominant in Greek, Roma and immigrant infants and children hospitalized in Greece. Thus, the predominant MLVA genotype in Greece is similar to other countries using acellular vaccines.<\/jats:p>","DOI":"10.1099\/jmm.0.000688","type":"journal-article","created":{"date-parts":[[2018,2,2]],"date-time":"2018-02-02T15:13:22Z","timestamp":1517584402000},"page":"400-407","source":"Crossref","is-referenced-by-count":7,"title":["Molecular epidemiology of Bordetella pertussis in Greece, 2010\u20132015"],"prefix":"10.1099","volume":"67","author":[{"given":"Evangelia","family":"Petridou","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, \u2018Aghia Sophia\u2019 Children's Hospital, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel Barker","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Virus and Microbiological Special Diagnostics, Parasites & Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanasios","family":"Arvanitidis","sequence":"additional","affiliation":[{"name":"Department of Virus and Microbiological Special Diagnostics, Parasites & Fungi, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Department of Bacteria, Parasites & Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Giannaki-Psinaki","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, \u2018Aghia Sophia\u2019 Children's Hospital, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanasios","family":"Michos","sequence":"additional","affiliation":[{"name":"First Department of Pediatrics, National and Kapodistrian University of Athens, \u2018Aghia Sophia\u2019 Children's Hospital, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Angeliki","family":"Krogfelt","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites & Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randi F\u00f8ns","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Virus and Microbiological Special Diagnostics, Parasites & Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268813000071"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1086\/652281"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/303846"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000160914.59160.41"},{"key":"R5","article-title":"Vaccine-preventable diseases: surveillance systems in Greece and epidemiological data","author":"Georgakopoulou","year":"2011"},{"key":"R6","article-title":"National study of vaccination coverage in children in Greece","author":"Panagiotopoulos","year":"2012"},{"key":"R7","article-title":"Epidemiological data for pertussis in Greece 2004-2014","year":"2015"},{"key":"R8","article-title":"Index mundi","year":"2017"},{"key":"R9","article-title":"Epidemiology of pertussis in a tertiary pediatric hospital in Athens, 2010\u2013 2012. 31st Annual Meeting of the European Society for Paediatric Infectious Diseases, Milan, Italy, May 28-June 1, 2013, Abstract no. 179","author":"Georgakopoulou"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.09.084"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1314688110"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.01.003"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.049585-0"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.16.5496-5505.2004"},{"key":"R15","article-title":"National Institute for Public Health and the Environment, Ministry of Health, Welfare and Sport","year":"2017"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.3201\/eid1805.110812"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2297-2"},{"key":"R18","first-page":"102","article-title":"Multiple-locus variable number tandem repeat analysis of Bordetella pertussis strains collected in the Czech Republic in 1967\u20132015: spread of a variant adapted to the population with a high vaccination coverage","volume":"65","author":"L\u017ei\u010da\u0159ov\u00e1","year":"2016","journal-title":"Epidemiol Mikrobiol Imunol"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis178"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077165"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.3201\/eid1808.120082"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000408"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2015.04.001"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2655-3"},{"key":"R25","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1128\/jcm.28.9.1982-1987.1990","article-title":"Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction","volume":"28","author":"Glare","year":"1990","journal-title":"J Clin Microbiol"},{"key":"R26","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1128\/jcm.31.8.2134-2140.1993","article-title":"Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis","volume":"31","author":"van der Zee","year":"1993","journal-title":"J Clin Microbiol"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1994.tb04878.x"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.3201\/eid1602.081707"},{"key":"R29","article-title":"Simpson\u2019s index of diversity. Comparing Partitions","year":"2011"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132623"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1038\/ng1227"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2837-2843.2005"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2009.06.019"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01838-08"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.000688?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T11:29:41Z","timestamp":1702639781000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.000688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,1]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.000688","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,1]]}}}