{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T04:36:34Z","timestamp":1648614994344},"reference-count":30,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,1,1]]},"abstract":"<jats:p>Chlamydial infection of the upper genital tract after abortion is well recognized, but routine screening for infection before termination is rare, and few centres are aware of the prevalence of post-abortion complications in their patient population. Knowledge of the patient population is the best guide for developing screening strategies. The aim of this study was to determine the prevalence of chlamydial infection in patients presenting for legal termination of pregnancy, and to assess the presence of <jats:italic>Chlamydia trachomatis<\/jats:italic> by PCR on specimens collected in either PreservCyt (ThinPrep) or 2-sucrose phosphate (2-SP) transport medium. Two hundred and eleven single, sexually active women, aged 15\u201326 years, attending the Gynaecology and Obstetric Hospital, Amiens, France, for surgical termination of pregnancy were enrolled in this study from June 2002 to June 2003. <jats:italic>C. trachomatis<\/jats:italic> detection using a Cobas Amplicor PCR test (Roche Diagnostics) targeting a 207\u2005bp segment of the common cryptic plasmid and a quantitative LightCycler real-time PCR (LC-PCR) (Roche Diagnostics) targeting a 123\u2005bp fragment within the highly conserved constant domain 3 of the single-chromosome-copy <jats:italic>ompA<\/jats:italic> gene were performed on endocervical swabs in 2-SP, and on specimens collected using a cytobrush and placed in PreservCyt medium. The in-house LC-PCR was used as a chromosomal diagnosis method and to determine the load of <jats:italic>C. trachomatis<\/jats:italic>. This method was able to detect the mutant Swedish variant with a deletion of 377\u2005bp in the target area in the cryptic plasmid, which is the region targeted by the Cobas Amplicor PCR test. <jats:italic>C. trachomatis<\/jats:italic> was detected in 19\/211 patients (9\u200a%) by both PCR methods. Among the 19 infected women, <jats:italic>C. trachomatis<\/jats:italic> was detected by the Cobas Amplicor PCR in 16 specimens in PreservCyt (7.6\u200a%) and in 12 endocervical swabs in 2-SP (5.7\u200a%). Specimens from only nine women were PCR-positive in both PreservCyt and 2-SP media by this method. Cobas Amplicor PCR revealed that 10.9 and 2.3\u200a% of the PreservCyt and 2-SP samples, respectively, contained inhibitors. The same 19 infected women were LC-PCR positive in both PreservCyt and 2-SP samples. No additional infected women were found by this last method; thus, it was concluded that none of the samples contained the new variant of <jats:italic>C. trachomatis<\/jats:italic>. The load in each sample varied from 10<jats:sup>2<\/jats:sup> to 10<jats:sup>7<\/jats:sup>\u2005copies\u2005ml<jats:sup>\u22121<\/jats:sup>.<\/jats:p>","DOI":"10.1099\/jmm.0.000737-0","type":"journal-article","created":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T19:33:05Z","timestamp":1229110385000},"page":"59-64","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of Chlamydia trachomatis infection by Cobas Amplicor PCR and in-house LightCycler assays using PreservCyt and 2-SP media in voluntary legal abortions"],"prefix":"10.1099","volume":"58","author":[{"given":"Henri","family":"Sevestre","sequence":"first","affiliation":[{"name":"Laboratoire d'Anatomo-Pathologie, Centre Hospitalo-Universitaire d'Amiens, Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Mention","sequence":"additional","affiliation":[{"name":"Centre de Gyn\u00e9cologie-Obst\u00e9trique, Centre Hospitalo-Universitaire d'Amiens, Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Fran\u00e7ois","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Centre de Ressources R\u00e9gionales en Biologie Mol\u00e9culaire, Universit\u00e9 de Picardie Jules Verne, Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Eb","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farida","family":"Hamdad","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","first-page":"52","article-title":"Chlamydia : a major health threat to adolescents and young 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