{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:13:57Z","timestamp":1790108037652,"version":"4.0.1"},"reference-count":29,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,1,1]]},"abstract":"\n Shigella<\/jats:italic> is the cause of shigellosis or bacillary dysentery, the occurrence of which is estimated to be 165 million cases per year worldwide, resulting in 1.1 million deaths. Rapid and reliable assays for detecting and identifying Shigella<\/jats:italic> in food, environmental and clinical samples are therefore necessary. Shigella<\/jats:italic> species are traditionally identified by their O antigens. This study developed a DNA microarray targeting O-serotype-specific genes to detect all 34 distinct O-antigen forms of Shigella<\/jats:italic>, including Shigella boydii<\/jats:italic> types 1\u201318, Shigella dysenteriae<\/jats:italic> types 1\u201313, Shigella flexneri<\/jats:italic> types 1\u20135 and 6, and Shigella sonnei<\/jats:italic>. A total of 282 strains were used to test the specificity of the microarray, including 186 Shigella<\/jats:italic> and Escherichia coli<\/jats:italic> representative strains, 86 Shigella<\/jats:italic> clinical isolates and ten strains of other bacterial species that are commonly isolated from food or clinical stool specimens. The oligonucleotide probes were printed on the microarray in concentrations from 1 to 100\u2005\u03bcM, and 10\u2005\u03bcM proved to be the optimal probe concentration. The detection sensitivity for each serotype was 50\u2005ng genomic DNA or 1 c.f.u. in 25\u2005g milk powder sample following a 6\u2005h enrichment in broth. The microarray is specific, sensitive and reproducible, and, to our knowledge, is the first report of a microarray for serotyping all O-antigen forms of Shigella<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.000794-0","type":"journal-article","created":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T19:33:05Z","timestamp":1229110385000},"page":"69-81","source":"Crossref","is-referenced-by-count":56,"title":["Molecular detection of all 34 distinct O-antigen forms of Shigella"],"prefix":"10.1099","volume":"58","author":[{"given":"Yayue","family":"Li","sequence":"first","affiliation":[{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"Tianjin Biochip Corporation, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"Tianjin University of Science and Technology, Tianjin 300457, PR China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyang","family":"Cao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, PR China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, PR China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 Hongda Street, TEDA, Tianjin 300457, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Biochip Corporation, 23 Hongda Street, TEDA, Tianjin 300457, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qili","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300457, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Peng","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Bastin","sequence":"additional","affiliation":[{"name":"Tianjin Biochip Corporation, 23 Hongda Street, TEDA, Tianjin 300457, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Feng","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Research Center for Functional 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