{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:25:30Z","timestamp":1790799930802,"version":"4.1.0"},"reference-count":65,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,1]]},"abstract":"\n Purpose<\/jats:bold>. This study aimed to characterize 27 \n \n Escherichia coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates obtained from peritoneal dialysis (PD)-related peritonitis that occurred at the University Hospital of Botucatu Medical School, Brazil, between 1997 and 2015.<\/jats:p>\n \n Methodology<\/jats:bold>. These isolates were characterized regarding the occurrence of 22 virulence factor-encoding genes, antimicrobial resistance and biofilm production. We then evaluated whether these factors influenced the clinical outcome.<\/jats:p>\n \n Results<\/jats:bold>. Over an 18-year period, 726 episodes of PD-related peritonitis were diagnosed, with 27 of them (3.7\u200a%) being due to \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The majority of the isolates were classified in phylogroups B1 (33.3\u200a%), B2 (30.0\u200a%) or F (18.0\u200a%). fimH<\/jats:italic> (100.0\u200a%), ompT<\/jats:italic> (66.7\u200a%) and irp2<\/jats:italic> (51.9\u200a%) were the most prevalent genes, while papA<\/jats:italic>, papC, iha, sat, irp2, iucD<\/jats:italic>, ireA<\/jats:italic>, ibe10, ompT<\/jats:italic> and kpsMTII<\/jats:italic> were significantly more prevalent among isolates belonging to phylogroups B2 and F (P<\/jats:italic><0.05). Non-susceptibility to quinolones was detected in six isolates, which harboured chromosomal and\/or plasmid-mediated quinolone resistance determinants, while two CTX-M extended-spectrum \u03b2-lactamase-producing \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were identified. Virulence factor-encoding genes (alone or in combination) and antimicrobial resistance were not associated with non-resolution outcomes. However, there was a trend for the ability to produce biofilm to be associated with treatment failure, although this association was not statistically significant.<\/jats:p>\n \n Conclusion<\/jats:bold>. The \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates were heterogeneous in terms of the features investigated, and were susceptible to most of the antimicrobial drugs tested, despite the unsuccessful treatment observed in more than 50.0\u200a% of the patients. Studies including more cases could help to clarify if biofilm production can influence the outcome in patients with PD-related peritonitis.<\/jats:p>","DOI":"10.1099\/jmm.0.001043","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T16:12:53Z","timestamp":1564157573000},"page":"1330-1340","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of Escherichia coli obtained from patients undergoing peritoneal dialysis and diagnosed with peritonitis in a Brazilian centre"],"prefix":"10.1099","volume":"68","author":[{"given":"Regiane C. 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