{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:20:10Z","timestamp":1783570810083,"version":"3.55.0"},"reference-count":17,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"\n Introduction.<\/jats:bold> Transmission of Enterobacterales in neonatal intensive care units (NICU) can cause outbreaks of colonization and invasive infections among neonates. Two clusters of nosocomial transmission of \n \n Klebsiella pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> identified by MALDI-ToF mass-spectrometry were suspected at two NICUs in July and August 2016.<\/jats:p>\n \n Aim.<\/jats:bold> To assess the potential transmission of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> among neonates.<\/jats:p>\n \n Methodology.<\/jats:bold> Whole-genome sequencing (WGS) was performed of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates obtained through targeted surveillance of patients and environmental sampling.<\/jats:p>\n \n Results.<\/jats:bold> WGS data revealed that patient and environmental isolates represented two species, \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Core-genome multi-locus sequence typing (cgMLST) of the isolates identified three separate transmission clusters, in Hospital A a cluster of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates in 12 children and two environmental samples and a second cluster of \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates in five children. In Hospital B a cluster of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates from three children and five unrelated isolates of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and two unrelated isolates of \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were found.<\/jats:p>\n \n Conclusion.<\/jats:bold> \n \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> can cause hospital outbreaks of colonization and infection similar to other \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp.<\/jats:p>\n Preliminary results from this study were presented at the 27th European Congress of Clinical Microbiology and Infectious Diseases, April 22-25, 2018, Vienna, Austria.<\/jats:p>","DOI":"10.1099\/jmm.0.001143","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T18:10:13Z","timestamp":1583259013000},"page":"396-401","source":"Crossref","is-referenced-by-count":26,"title":["Klebsiella variicola causing nosocomial transmission among neonates \u2013 an emerging pathogen?"],"prefix":"10.1099","volume":"69","author":[{"given":"Ellen","family":"Piepenbrock","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8677-9454","authenticated-orcid":false,"given":"Paul G.","family":"Higgins","sequence":"additional","affiliation":[{"name":"German Center for Infection Research (DZIF), Partner site Bonn-Cologne, Germany"},{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, 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