{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:02:14Z","timestamp":1769072534353,"version":"3.49.0"},"reference-count":21,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["18fk0108049j1902"],"award-info":[{"award-number":["18fk0108049j1902"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["19fk0108049j1903"],"award-info":[{"award-number":["19fk0108049j1903"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:p>Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7893\" xlink:type=\"simple\">Leptospira<\/jats:ext-link><\/jats:named-content><\/jats:italic>species. It is a public health issue in the tropics, including Okinawa, the southernmost prefecture of Japan. This study reports the first isolation of<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7894\" xlink:type=\"simple\">L. interrogans<\/jats:ext-link><\/jats:named-content><\/jats:italic>serogroup Sejroe from two human patients in Japan, and describes its molecular characterization using multilocus sequence typing (MLST) and multiple-locus variable-number tandem repeat analysis (MLVA). MLST on the two isolates, 168036 and 178129, showed that<jats:italic>pfkB<\/jats:italic>in 178129 is a novel allele, and that both isolates constitute novel sequence types (STs); ST286 for 168036 and ST287 for 178129. A minimum spanning tree based on seven alleles of<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7894\" xlink:type=\"simple\">L. interrogans<\/jats:ext-link><\/jats:named-content><\/jats:italic>indicates that both isolates are genetically close, but are distinct from known<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7894\" xlink:type=\"simple\">L. interrogans<\/jats:ext-link><\/jats:named-content><\/jats:italic>serogroup Sejroe strains. MLVA using 11 loci demonstrated that seven of the 11 loci were identical between the two isolates, whereas the identity between the isolates and the seven reference strains of<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7894\" xlink:type=\"simple\">L. interrogans<\/jats:ext-link><\/jats:named-content><\/jats:italic>serogroup Sejroe was zero to three loci. These results indicate that the isolates investigated in this study have novel genotypes, and are genetically closest to each other among the known<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7894\" xlink:type=\"simple\">L. interrogans<\/jats:ext-link><\/jats:named-content><\/jats:italic>serogroup Sejroe strains.<\/jats:p>","DOI":"10.1099\/jmm.0.001169","type":"journal-article","created":{"date-parts":[[2020,5,10]],"date-time":"2020-05-10T22:12:21Z","timestamp":1589148741000},"page":"587-590","source":"Crossref","is-referenced-by-count":4,"title":["Novel genotypes of Leptospira interrogans serogroup Sejroe isolated from human patients in Okinawa Prefecture, Japan"],"prefix":"10.1099","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8991-7397","authenticated-orcid":false,"given":"Tetsuya","family":"Kakita","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisako","family":"Kyan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Miyahira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taketoshi","family":"Takara","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eri","family":"Nakama","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumani","family":"Kuba","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Nidaira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Kudaka","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5733-535X","authenticated-orcid":false,"given":"Nobuo","family":"Koizumi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Disease, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.06.009"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007374"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007270"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"R6","volume-title":"Leptospira and Leptospirosis","author":"Faine","year":"1999"},{"key":"R7","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/978-3-662-45059-8_6","volume-title":"Adler B. 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