{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T02:29:40Z","timestamp":1774060180738,"version":"3.50.1"},"reference-count":28,"publisher":"Microbiology Society","issue":"6","funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["305024\/2017-5"],"award-info":[{"award-number":["305024\/2017-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Sporotrichosis, caused by species of the <jats:italic>Sporothrix schenckii<\/jats:italic> complex, is the most prevalent subcutaneous mycosis in many areas of Latin America. Statins are a class of drugs widely used for lowering high sterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme in the synthesis of sterol.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> In this study, the antifungal activity of statins (simvastatin, atorvastatin, pravastatin) against planktonic cells and biofilms of <jats:italic>S. schenckii<\/jats:italic> complex species was evaluated, as well as the interaction of pravastatin with classical antifungals (amphotericin B, itraconazole, terbinafine).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Eighteen strains of <jats:italic>Sporothrix<\/jats:italic> species were used. The antifungal susceptibility assay was performed using the broth microdilution method. Mature biofilms were exposed to statins and metabolic activity was measured by the XTT reduction assay.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> MICs of statins ranged from 8 to 512 \u03bcg ml<jats:sup>\u22121<\/jats:sup> and from 8 to 256 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for filamentous and yeast forms, respectively. Regarding mature biofilms, MICs of 50\u200a% inhibition (SMIC50) were 128 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for simvastatin and atorvastatin and &gt;2048 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for pravastatin. MICs of 90\u200a% inhibition (SMIC90) were 512 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for simvastatin and &gt;2048 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for atorvastatin and pravastatin.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion<\/jats:bold>. These results highlight the antifungal and antibiofilm potential of statins against <jats:italic>S. schenckii<\/jats:italic> complex species.<\/jats:p>","DOI":"10.1099\/jmm.0.001195","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T15:18:21Z","timestamp":1589901501000},"page":"838-843","source":"Crossref","is-referenced-by-count":5,"title":["In vitro inhibitory effect of statins on planktonic cells and biofilms of the Sporothrix schenckii species complex"],"prefix":"10.1099","volume":"69","author":[{"given":"Raimunda S\u00e2mia Nogueira","family":"Brilhante","sequence":"first","affiliation":[{"name":"Specialized Center in Medical Mycology, Postgraduate Program in Medical Microbiology, Department of Pathology and Forensic Medicine, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, 60430-275, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xhaulla Maria Quariguasi 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