{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:28:22Z","timestamp":1762918102560,"version":"3.40.5"},"reference-count":31,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"Japanese Society for the Promotion of Science Grant-in-Aid for Scientific Research","award":["25860825"],"award-info":[{"award-number":["25860825"]}]},{"name":"Japanese Society for the Promotion of Science Grant-in-Aid for Scientific Research","award":["18K08424"],"award-info":[{"award-number":["18K08424"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"\n Introduction.<\/jats:bold> \n Bacillus cereus <\/jats:italic>harbouring Ba813<\/jats:italic>, a specific chromosomal marker of Bacillus anthtacis,<\/jats:italic> is found in patients with severe manifestations and causes nosocomial outbreaks.<\/jats:p>\n \n Aim.<\/jats:bold> We assessed the genetic characteristics and virulence of Ba813<\/jats:italic>(+) \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in a hospital setting.<\/jats:p>\n \n Methodology.<\/jats:bold> Three neutropenic patients with haematological malignancy developed \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> bacteraemia within a short period. Fifteen \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were isolated from different sites in a haematology ward. A total of 18 isolates were evaluated for Ba813<\/jats:italic>- and \n \n B. anthracis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-related virulence, food poisoning-related virulence, genetic diversity, bacteria motility and biofilm formation.<\/jats:p>\n \n Results.<\/jats:bold> \n Ba813<\/jats:italic>(+) \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> was detected in 33\u200a% (1\/3) of patients and 66\u200a% (9\/15) of the hospital environment. The 18 strains were divided into 2 major clusters (clade 1 and clade 2), and 14 strains were classified into clade 1. All Ba813<\/jats:italic>(+) strains, including four sequence types, were classified into clade 1\/the cereus III lineage, which is most closely related to the anthracis lineage. Two strains belonging to clade 1\/non-cereus III carried the \n \n B. anthracis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-associated cap<\/jats:italic> gene, but not Ba813. B. cereus<\/jats:italic>, including Ba813<\/jats:italic>(+) strains, had significantly lower prevalence of enterotoxin genes than clade 2 strains. In clade 1, \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, Ba813<\/jats:italic>(+) strains showed significantly higher swimming motility and biofilm formation ability than Ba813<\/jats:italic>(\u2212) strains.<\/jats:p>\n \n Conclusion.<\/jats:bold> \n Ba813<\/jats:italic>(+) \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, which are genetically closely related to \n \n B. anthracis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, were abundant in a haematological ward. Ba813<\/jats:italic>(+) \n \n B. cereus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with high motility and biofilm formation abilities may spread easily in hospital environments, and could become a hospital-acquired infection.<\/jats:p>","DOI":"10.1099\/jmm.0.001212","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T15:15:20Z","timestamp":1591974920000},"page":"999-1004","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of Ba813 harbouring Bacillus cereus in patients with haematological malignancy and hospital environments at a medical centre in Japan"],"prefix":"10.1099","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6821-9701","authenticated-orcid":false,"given":"Tetsuji","family":"Aoyagi","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Internal Medicine, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aobaku, Sendai, 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